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How does a smear preparation of cells from a liquid medium differ from preparation of cells from a solid medium?

In liquid medium one starts by placing one or two loopfuls of the liquid medium directly on the slide. From solid media such as nutrient agar. One starts by placing one or two loopfuls of water on the slide and then using an inoculation loop to disperse the organisms in the water. Bacteria growing on solkid media tend to cling to each other and mus be dispensed suficiently by dilutiion in water.


What happens to Smudge cells in an albumin smear?

Cell fragility causes smudge cells to appear on peripheral smears. Addition of albumin prior to slide preparation essentially coats the fragile cells, thus keeping them intact on smear preparation.


Do cells in the G0 phase synthesize DNA?

No. DNA synthesis occurs in the S phase. Cells in the G0 phase are not preparing for cell division.


Why use iodine when preparing slide for viewing?

one disadvantage of using iodine to stain cells is that iodine will kill living cells.


What substance is used to arrest a cell in metaphase for karyotype preparation?

Colchicine is commonly used to arrest cells in metaphase for karyotype preparation. Colchicine inhibits microtubule formation, preventing cells from progressing through mitosis and causing arrest in metaphase.


How do you prepare the cells when you want to use them for chromosome preparation?

To prepare cells for chromosome preparation, first culture the cells to reach optimal growth and then arrest them at a specific stage within the cell cycle. Following this, the cells are treated with a hypotonic solution to swell and burst the cells, releasing the chromosomes. The chromosomes can then be fixed and stained for visualization under a microscope.


What cell is important to cells in the heart?

The most important cells are the cardiac muscles cells that do the pumping.


What do cells spend most of their time doing?

Interphase, due to the fact that it is preparing its self for cell division.


Why use dye in preparation of cheek smear specimen?

Dye is used in the preparation of cheek smear specimens to provide contrast and make it easier to visualize and identify cells under the microscope. Dye helps highlight the cellular structures of the cheek cells, making it easier for scientists to analyze and study them.


Why is cell important?

Cells are important because everything is basically made of cells.


What are the two procedures used to obtain the cells needed for preparing a fetal karyotype?

The two procedures used to obtain cells for preparing a fetal karyotype are chorionic villus sampling (CVS) and amniocentesis. CVS involves taking a sample of cells from the placenta, while amniocentesis involves collecting cells from the amniotic fluid surrounding the fetus.


What phase are most cells in when they stop dividing?

In between divisions cells enter interphase. During interphase the cell is growing in preparation for the next division. Cells that are no longer dividing are considered to be in senescence. Senescent cells are not growing or replicating their DNA.