A dense smear will make it difficult to evaluate cells, as they may be all "piled up" and hard to evaluate.
because if too much smear the sample will look to indistinct
bcoz a heat-fix smear have a good contrast than the wet mount
Yes, when you are performing smear preparation, you should always use sterilized water. This will ensure that nothing contaminates your slide.
no sign from the teacher.
Thick or dense smears contain too many cells that can overlap, making it difficult to see individual cells clearly under the microscope. This can lead to inaccurate interpretation of the sample. A thinner smear allows for better visualization of individual cells and structures.
Cell fragility causes smudge cells to appear on peripheral smears. Addition of albumin prior to slide preparation essentially coats the fragile cells, thus keeping them intact on smear preparation.
Tzanck preparation is also called a Tzanck smear, herpes stain for inclusion bodies, or inclusion bodies stain.
smear means that when you smooth something it means the same thing.
no thing
Dye is used in the preparation of cheek smear specimens to provide contrast and make it easier to visualize and identify cells under the microscope. Dye helps highlight the cellular structures of the cheek cells, making it easier for scientists to analyze and study them.
During the preparation of a bacterial smear, it is crucial to use sterile techniques to avoid contamination. Use a sterile inoculating loop to transfer a small amount of culture onto a clean slide, and ensure the smear is thin and evenly spread. When heat-fixing, gently pass the slide through a flame to adhere the bacteria to the slide without burning them, as excessive heat can distort cellular structures. Always wear gloves and goggles to protect yourself from potential biohazards.
The smear will not adhere well if there is grease on the slide.