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Over destaining can cause the purple color from the gram positive bacteria to be washed away, so when you apply the safrinin the gram positive bacteria will take up a pink color causing gram positive bacteria to appear gram negative.

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11y ago
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13y ago

You can't overdecolorize gram negative organisms. Gram negative are colorless regardless.

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Q: What would be the result of over destaining on gram-positive types?
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