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When DNA fragments from different sources are mixed together they begin combining with each other due to?
Wiki User
∙ 11y ago
Recombination. You can "stitch" together template DNA fragments from differing sources by combining them in a buffered master mixture containing dNTPS, polymerase, and specific primers that bind at the 5' end of Fragment1 and a primer complementary to the 3' end of Fragment2. After running through a thermocycler, under the right conditions, the primers will bind and replicate the strands. If the strands have sequences that overlap at the 3' end of Frag1 and the 5' end of Frag2, then replication will "stitch" these two fragments together. There is also non-homologous end-joining in which the aforementioned sequences do not need to overlap, however I'm an undergrad student who just started conducting biomedical research in this area and haven't worked with NHEJ. My current work uses fragments with overlapping sequences as mentioned, and this is to my current understanding, so don't take my word for complete fact until you perform the due diligence yourself on the subject.
Wiki User
∙ 11y ago
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DNA molecules that are produced by combining DNA from different sources are called?
The word you're looking for may be "recombinant".
How are the cleaved DNA fragments from two sources able to recombine?
Sometimes, when the cleaved DNA fragments both have sticky ends, they naturally anneal due to complementary base pairing. However, an enzyme called DNA Ligase is used for joining cut strands of DNA together. There is a ligase called t4 Ligase that joins blunt ends because it is stronger.
What is a DNA molecule containing regions from different sources?
Chimeras. In genetic engineering, molecules of combined DNA are known as chimeras because they are produced by combining DNA from different species. Combined DNA is also known as recombinant DNA, since DNA from 2 sources has been recombined to produce it.
The strategy of combining two incomplete plant protein sources so that the amino acids in one food make up for those lacking in the other food is called?
Protein combining, sometimes called protein complementing, is often practiced by vegetarians in order to ensure optimal metabolic performance. The concept was originally utilized in animals, but became more popular in human dieting in the 1970s.
What tissue type is the epiphyseal plate composed?
Cartilage druing youth, then they fuse together after adolescence. Sources: I have an A in physiology:)
What is produced by combining genes from different sources?
When combining genes from different sources, whether it be in the plant or animal kingdoms the outcome will result in what is known as a hybrid, or new species with the potential to carry the genes of both original specimens.
What technology involves combining segments of DNA from different sources to form a single DNA molecule?
sexual reproduction
What is the process of taking signals from different sources and combining them to send over one cable called?
asteroids! Multiplexing
DNA molecules that are produced by combining DNA from different sources are called?
The word you're looking for may be "recombinant".
What happens when two waves from different sources come together at a single point?
Interference
What energy sources produces electricity by chemically combining gases or liquids into fuel?
fuel cells
Why does one need a data integration platform?
Data integration involves combining data residing in different sources and providing users with a unified view of these data. This process becomes significant in a variety of situations, which include both commercial (when two similar companies need to merge their databases) and scientific (combining research results from different bioinformatics repositories, for example) domains.
How are the cleaved DNA fragments from two sources able to recombine?
Sometimes, when the cleaved DNA fragments both have sticky ends, they naturally anneal due to complementary base pairing. However, an enzyme called DNA Ligase is used for joining cut strands of DNA together. There is a ligase called t4 Ligase that joins blunt ends because it is stronger.
How is rcombinant DNA made?
Recombinant DNA is made by combining DNA from different sources. The most common way is to use a plasmid (a circular piece of prokaryote DNA), cut it with a restriction enzyme and then mix it with your target gene which has also been cut with the same enzymes so the pieces fit together like a jigsaw puzzle.
Why different sources of finance have different cost?
why different sources of financing have different costs
Why different sources of finance have different costs?
why different sources of financing have different costs
What is the process which technology involves combining segments of DNA from differebt sources to form a single DNA?
Marc john
