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How are the cleaved DNA fragments from two sources able to recombine?
Wiki User
∙ 13y ago
Sometimes, when the cleaved DNA fragments both have sticky ends, they naturally anneal due to complementary base pairing. However, an enzyme called DNA Ligase is used for joining cut strands of DNA together. There is a ligase called t4 Ligase that joins blunt ends because it is stronger.
Wiki User
∙ 13y ago
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During gel electrophoresis do long or short fragments travel more quickly toward the positive pole?
Short fragments are able to move faster (longer = heavier = slower).
If you have a restriction enzyme that cuts a piece of DNA at two recognition sites how many DNA fragments would you see on a gel?
Three.To see why, cut a piece of string in two places! Of course, strictly you would not be able to see only three fragments. You would amplify the DNA before carrying out electrophoresis. That way, you would get perhaps 200 million copies of each fragment, and they would show up. Also, you would only be able to distinguish the fragments if they were different lengths. Electrophoresis separates pieces of DNA by length.
How do you interpret electrophoresis?
In electrophoresis, DNA is subjected to an electric field which causes the genetic material to migrate in a direction from a cathode to an anode. The DNA that is closest to the anode is determined to be shorter in length compared to the DNA that is closer to the anode. This is explained by fact that the smaller fragments of DNA are better able to travel through the porous gel.
Humans raccoons and bears are omnivores - what adaptive advantage might this feeding strategy provide?
Omnivores have an adaptive advantage. This is because they are able to eat two different food sources in case one disappears.
What are the short segments produced in the formation of the 3'-5' strand of DNA called?
During DNA replication, the lagging strand is replicated ~1000 (E. coli) base pairs at a time, forming numerous "Okazaki fragments".Okazaki fragments form because polymerase is only able to replicate DNA in one direction, but DNA is double stranded, with the strands running anti parallel (in opposite directions). The polymerase waits for a region of DNA to be unwound, and while the leading strand is replicated continuously, on the lagging strand the polymerase waits until a region of single stranded DNA is produced before replicating it. This discontinous replication forms the Okazaki fragments, which can then be joined together by ligase (although a different polymerase enzyme, pol I in E. coli, is needed as well to replace the RNA primers with DNA).
Why are sources important for historians?
You need sources to be able to unravel history.
Why are D1S80 fragments separated by gel electophoresis?
Fragments are separated by gel electrophoresis because of their differing sizes. DNA is negatively charged, so will migrate through the gel towards the positive electrode. The smaller fragments are able to move through the gel more quickly than the larger fragments - which means they separate based on their size.
What kind of pollution comes from track able sources?
The kind of pollution comes from track able sources is usually trash pollution in the water. Air pollution and fossil fuel pollution are also track able sources.
During gel electrophoresis do long or short fragments travel more quickly toward the positive pole?
Short fragments are able to move faster (longer = heavier = slower).
What would happen to your bodies if it had no energy sources?
You'd die. You wouldn't be able to survive without energy sources.
When isolating genomic DNA how were you able to determine that the DNA was composed of large pieces and not small fragments?
Run them through a gel electrophoresis machine against a standard marker that would tell you the length of the fragments, thus large pieces from smaller pieces.
What are the quantities of a good researcher?
Being able to hide your sources ;)
What is the purpose of the slate rock?
Slate is a fine-grained, foliated, homogeneous metamorphic of silt. It is able to be cleaved into sheets which can be used as roofing slates, or left thicker to form hard wearing paths, in house building, ornamental fireplaces, and so on.
Why cydia sources do not work?
Cydia sources are essentially a website that is understood by Cydia. So, if the website is down, you will not be able to access the source and its packages.
Why is it necessary in todays world to use non conventional sources of energy?
we need these sources because these sources are recycle-able. This fact makes non conventional sources are most beneficial and useful. the conventional sources are very rare in this word and nearly will finished and these can't recycle.
What are the bedbug's advantages to living in beds?
They are able to be close to their food sources. (Humans)
Where would a person be able to find Lenox Poppies on Blue?
One is able to fine Lenox Poppies on Blue from a variety of online sources. Such sources include eBay, the Pronto, Next Tag, Price Machine, ans Shopzilla.
