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During gel electrophoresis do long or short fragments travel more quickly toward the positive pole?
Wiki User
∙ 12y ago
Short fragments are able to move faster (longer = heavier = slower).
Wiki User
∙ 12y ago
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Properties of DNA fragments which allow them to be separated from each other during gel electrophoresis?
They are negatively charged and are of different sizes
Why all DNA molecules move in the same direction in an electric field?
DNA molecules are negatively charged. When places in an electric field, like in an gel during the process of electrophoresis, they all move toward the positive electrode.
When the nucleus disappears during prophase it fragments into which fuse to reform the nucleus during telophase?
The nuclear envelope disappears during prometaphase.
DNA fragments from a gel are transferred to a nitrocellulose paper during the procedure called Southern blotting What is the purpose of transferring the DNA from a gel to a nitocellulose paper?
to permanently attach the DNA fragments to a substrate.
During DNA replication the short sections of new DNA known as okazaki fragments which are eventually linked together by ligase?
lagging
Which fragements travel the fastest and farthest during electrophoresis?
The smallest and lightest fragments.
Properties of DNA fragments which allow them to be separated from each other during gel electrophoresis?
They are negatively charged and are of different sizes
What moves farther shorter or taller fragments Why?
Shorter fragments typically move farther during gel electrophoresis because they experience less resistance in the gel matrix, allowing them to travel more quickly through the electric field. Taller fragments are larger and encounter more obstacles, causing them to move slower and remain closer to the origin.
What holds the DNA sample during electrophoresis?
DNA samples are within the gel matrix during electrophoresis. DNA moves at differtent rates through the pores of the gel depending on how long the fragments are. DNA is held by the gel itself.
When is DNA cut during electrophoresis?
Electrophoresis technique is not designed to cut DNA molecule. When DNA is analyzed by electrophoresis to determine its molecular mass, the molecular biology engineer usualy digests the DNA molecule, before the electrophoresis, with specific enzymes called "restriction enzymes" in order to obtain fragments of diverse molecular weights that can be seen as bands in electrophoresis gels.
What causes the bands on your DNA gels?
During gel electrophoresis, the DNA moves along the agarose gel to the positive side of the box, and after a certain amount of time, the smaller DNA fragments travel the farthest (because they have an easier time navigating the pores of the gel) and so on, leaving behind a series of bands comprised of similar-sized DNA fragments.
Do positively charged DNA molecules flow to the negatively charged poles in the electrophoresis chamber?
Yes. Positive(+) goes to negative(-). During gel electrophoresis, the positively charged molecules move to the negative cathode, and vis versa the negatively charged molecules move towards the positive anode.
Why do you need a molecular weight ruler alongside your samples during electrophoresis?
A molecular weight ruler uses a sample of fragments of a known size (known as a molecular weight marker) to be placed alongside the experimental and control samples. It helps compare the migration distance of the experimental fragments to the migrating distance of the fragments of a known size that make up the molecular weight marker. Then the scientist can calculate an approx. size of their experimental samples.
Why all DNA molecules move in the same direction in an electric field?
DNA molecules are negatively charged. When places in an electric field, like in an gel during the process of electrophoresis, they all move toward the positive electrode.
Some viva voce questions for electrophoresis?
1. WHAT IS ELECTROPHORESIS AND WHAT ARE THE IMPORTANTAPPLICATIONS OF ELECTROPHORESIS?Ans. Movement of charged particle in the electric field either towards cathode or anode whensubjected to an electric current is called electrophoresis.The following factors influence the movement of particles during the electrophoresis.(a) Electric current.(b) Net charge of the particle.(c) Size and shape of the particle.(d) Type of supporting media.(e) Buffer solution.Important Applications of ElectrophoresisThe technique of electrophoresis is used to separate and identify the(i) Serum proteins(ii) Serum lipoproteins(iii) Blood hemoglobins2. WHAT ARE THE DIFFERENT TYPES OF ELECTROPHORESIS?Ans. (a) Moving boundary electrophoresis: This technique was first introduced by TISELIUS in 1937(b) Zone electrophoresis: In this type of electrophoresis different types of supporting mediaare used. These are;(a) Paper electrophoresis(i) Whatman filter paper(ii) Cellulose acetate(b) Gel electrophoresis(i) Agarose.(ii) Polyacrylamide gel (used for the separation of isoenzymes).(iii) SDS-PAGE.(iv) Iso-electric focussing (proteins seperated in a medium possessing a stable pH gradient).(v) Immuno electrophoresis (for the separation of immunoglobulins).
What is the Working principal of electrophoresis?
Separates DNA fragments so they can be seen
Is during an volcanic eruption particles called lava ranging from very fine dust to pieces weighing several tons are ejected?
They are called fragments, fragments.
