0
Properties of DNA fragments which allow them to be separated from each other during gel electrophoresis?
Wiki User
∙ 12y ago
They are negatively charged and are of different sizes
Wiki User
∙ 12y ago
Add your answer:
Earn +20 pts
During gel electrophoresis do long or short fragments travel more quickly toward the positive pole?
Short fragments are able to move faster (longer = heavier = slower).
What structures are separated during anaphase 1?
The one of the two matching pairs of chromosomes are separated during anaphase I.
When the nucleus disappears during prophase it fragments into which fuse to reform the nucleus during telophase?
The nuclear envelope disappears during prometaphase.
DNA fragments from a gel are transferred to a nitrocellulose paper during the procedure called Southern blotting What is the purpose of transferring the DNA from a gel to a nitocellulose paper?
to permanently attach the DNA fragments to a substrate.
During DNA replication the short sections of new DNA known as okazaki fragments which are eventually linked together by ligase?
lagging
Which fragements travel the fastest and farthest during electrophoresis?
The smallest and lightest fragments.
What holds the DNA sample during electrophoresis?
DNA samples are within the gel matrix during electrophoresis. DNA moves at differtent rates through the pores of the gel depending on how long the fragments are. DNA is held by the gel itself.
When is DNA cut during electrophoresis?
Electrophoresis technique is not designed to cut DNA molecule. When DNA is analyzed by electrophoresis to determine its molecular mass, the molecular biology engineer usualy digests the DNA molecule, before the electrophoresis, with specific enzymes called "restriction enzymes" in order to obtain fragments of diverse molecular weights that can be seen as bands in electrophoresis gels.
During gel electrophoresis do long or short fragments travel more quickly toward the positive pole?
Short fragments are able to move faster (longer = heavier = slower).
What moves farther shorter or taller fragments Why?
Shorter fragments typically move farther during gel electrophoresis because they experience less resistance in the gel matrix, allowing them to travel more quickly through the electric field. Taller fragments are larger and encounter more obstacles, causing them to move slower and remain closer to the origin.
Where does the wastage of diamond goes?
Diamond fragments separated from a diamond stone during the cutting process are collected and used to cut and polish other diamonds.
Why do you need a molecular weight ruler alongside your samples during electrophoresis?
A molecular weight ruler uses a sample of fragments of a known size (known as a molecular weight marker) to be placed alongside the experimental and control samples. It helps compare the migration distance of the experimental fragments to the migrating distance of the fragments of a known size that make up the molecular weight marker. Then the scientist can calculate an approx. size of their experimental samples.
What causes the bands on your DNA gels?
During gel electrophoresis, the DNA moves along the agarose gel to the positive side of the box, and after a certain amount of time, the smaller DNA fragments travel the farthest (because they have an easier time navigating the pores of the gel) and so on, leaving behind a series of bands comprised of similar-sized DNA fragments.
What is the Working principal of electrophoresis?
Separates DNA fragments so they can be seen
What are the four functions of gel in gel electrophoresis?
There are several functions of the gel matrix in electrophoresis techniques. Four of the most important are:The gel cut down convection currents and diffusion so that the separated components remain as sharp zones with maximum resolution between them;the nature of the gel (in most cases is a network of acrilamide branches or polyacrilamide) is chemically inert during the separation porcess;it is clearly uniform in its properties and able to be readily and reproducibly prepared; andthe composition of the gel can be modified easily and in a controlled way varying the concengtrations of its components in order to achieve the best conditions for the problem at hand.
What is the purpose of ethidium bromide?
Ethidium bromide is an intercalating agent that attaches itself between the helix of a DNA. Because the ethidium molecule lights up when illuminated by an ultraviolet light, it is used often in biochemistry laboratories so that fragment of DNA that has been separated by gel can be visualized.
What structures are separated during anaphase 1?
The one of the two matching pairs of chromosomes are separated during anaphase I.
