0
What else can I help you with?
If you were called away during the electrophoresis procedure and were not able to monitor your sample run what would happen if the electricity were to remain running in your absence?
If the electrophoresis procedure continues unmonitored, the samples could run for too long, potentially causing the samples to run off the gel. This can lead to inaccurate results and loss of data. It is important to periodically check the progress of the electrophoresis run to ensure that the samples are running properly and do not get overextended.
Molecules are spaced farthest apart in?
Molecules are spaced farthest apart in the gaseous state because the particles have enough energy to move around and separate from each other. In a gas, the molecules are free to move in all directions and only come close together during collisions.
What is the fastest acceleration manned machine?
The fastest acceleration experienced by humans is typically during a rocket launch into space, with accelerations reaching up to 3-4 times the force of gravity (3-4 Gs). The Space Shuttle, for example, could reach accelerations of around 3 Gs during takeoff.
What is the fastest of the seismic waves?
p waves aka primary waves
Which process processes do the particles of water move the fastest?
Particles of water move the fastest during the process of evaporation, where they escape the liquid state and become vapor. This occurs when water is heated and the molecules gain enough energy to break free from the liquid surface.
Which pieces of DNA will migrate to the bottom of the DNA gel first during electrophoresis?
During electrophoresis, smaller pieces of DNA will migrate to the bottom of the gel first.
The results of gel electrophoresis are shown below with four different strands of DNA labeled. Which strand of DNA is the shortest?
Answer this q The results of gel electrophoresis are shown below, with four different strands of DNA labeled.Which strand of DNA is the shortest? uestion…
When is DNA cut during electrophoresis?
Electrophoresis technique is not designed to cut DNA molecule. When DNA is analyzed by electrophoresis to determine its molecular mass, the molecular biology engineer usualy digests the DNA molecule, before the electrophoresis, with specific enzymes called "restriction enzymes" in order to obtain fragments of diverse molecular weights that can be seen as bands in electrophoresis gels.
What is the function of comb in electrophoresis?
The comb is used to create wells in the gel where samples can be loaded for electrophoresis. It helps to organize the samples and ensure that they are separated properly during the process.
Where do you place the DNA samples on the gel during electrophoresis?
During electrophoresis, DNA samples are placed at the wells of the gel. The gel is then subjected to an electric current, causing the DNA fragments to move through the gel based on their size.
Why do DNA pieces migrate from the top of the gel towards the bottom during gel electrophoresis?
During gel electrophoresis, DNA pieces migrate from the top of the gel towards the bottom because they are negatively charged and are attracted to the positive electrode at the bottom of the gel.
What was the farthest German advance east during WW2?
The Ukraine was the farthest they got. See map on link below.
Why does DNA move through the gel during gel electrophoresis?
During gel electrophoresis, DNA moves through the gel because it is negatively charged and is attracted to the positive electrode. The DNA molecules are pulled through the gel by an electric field, separating them based on size.
What is the sun farthest north or south of the equator at this time?
The sun is farthest north of the Equator during the summer solstice, which occurs around June 21st each year, and farthest south during the winter solstice around December 21st.
Why is glycerol and blue dye added to the gel loading buffer during gel electrophoresis?
Glycerol is added to make the DNA sample denser so that it sinks into the gel and loads properly. Blue dye is added to visualize the sample loading and migration progress during electrophoresis.
What is the recommended well gel loading volume for optimal results in gel electrophoresis?
The recommended well gel loading volume for optimal results in gel electrophoresis is typically around 10-20 microliters. This volume helps ensure that the samples are loaded evenly and do not overflow or distort the gel during the electrophoresis process.
Some viva voce questions for electrophoresis?
1. WHAT IS ELECTROPHORESIS AND WHAT ARE THE IMPORTANTAPPLICATIONS OF ELECTROPHORESIS?Ans. Movement of charged particle in the electric field either towards cathode or anode whensubjected to an electric current is called electrophoresis.The following factors influence the movement of particles during the electrophoresis.(a) Electric current.(b) Net charge of the particle.(c) Size and shape of the particle.(d) Type of supporting media.(e) Buffer solution.Important Applications of ElectrophoresisThe technique of electrophoresis is used to separate and identify the(i) Serum proteins(ii) Serum lipoproteins(iii) Blood hemoglobins2. WHAT ARE THE DIFFERENT TYPES OF ELECTROPHORESIS?Ans. (a) Moving boundary electrophoresis: This technique was first introduced by TISELIUS in 1937(b) Zone electrophoresis: In this type of electrophoresis different types of supporting mediaare used. These are;(a) Paper electrophoresis(i) Whatman filter paper(ii) Cellulose acetate(b) Gel electrophoresis(i) Agarose.(ii) Polyacrylamide gel (used for the separation of isoenzymes).(iii) SDS-PAGE.(iv) Iso-electric focussing (proteins seperated in a medium possessing a stable pH gradient).(v) Immuno electrophoresis (for the separation of immunoglobulins).
