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When using streak plates, the colonies should appear along the streak lines. This is where the bacteria have been introduced and is the first place they will grow.

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Q: Where should colonies appear in the case of streak plates?
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Why are agar plates dried before being used for the preparation of dilution streak plates?

perhaps it is easier to streak that way, i mean when the agar is set and dry. .


If an inoculated plate had colonies between the streak lines what would you conclude?

It depends, if there is no growth or colony appearance on streak line and only it shows growth in b/w the streak line then it is certainly a contamination and if there are colonies on streak line and not ressemble with the streak culture then also it is a contamination but there can be a chance that colony appears due to some fault in streaking procedure and the inoculum drops between the streak line so it depends.


What advantage does the streak plate method have over the pour plate method?

A disadvantage of the streak plate technique could be colony isolation problems. If the streaking technique is not done properly or if there is too much of an organism present on the inoculating loop then the cells can cluster and form what looks like one colony but it can actually be a couple colonies (made from a couple cells). This can cause an inaccurate colony forming unit count.


Why use a streak plate to grow a bacterium?

You do a streak plate in order to get isolated colonies. If you inoculate into a slant, you have less surface area to work and less chance of getting isolated colonies. In broth, you'll definitely get growth but you won't know WHAT is growing. You go back into each quadrant (a little) with your loop in order to "dilute" the bacteria and get colonies. Quadrant 1 is pretty think (like a smear on the plate) but by the time you get to Quadrants 3 and 4, you should see more defined colonies and not just a film of bacteria.


Why does the streaking method you used to inoculate you plates result in isolated colonies?

In the streak plate technique, a loop is used to streak the mixed sample many times over the surface of a solid culture medium in a Petri plate. Theoretically, the process of streaking the loop repeatedly over the agar surface causes the bacteria to fall off the loop one by one and ultimately to be distributed over the agar surface, where each cell develops into a colony.

Related questions

Why are agar plates dried before being used for the preparation of dilution streak plates?

perhaps it is easier to streak that way, i mean when the agar is set and dry. .


What does the streak plate method have over the pour plate method?

It is more likely to give individual colonies regardless of the concentration of the original source. With pour plates, you might have to use several plates with different dilutions of inoculum to get individual colonies.


Why is the streak-stab technique preferred rather than incubating the plates anaerobically?

The streak-stab technique is preferred over incubating the plates anaerobically because when isolating colonies allows biochemical testing to be performed. When the plate is incubated anaerobically it lacks oxygen and can not be biochemically tested.


What is shale's streak?

Shale normally leaves a brown streak on unglazed porcelain plates. However, shale can also leave a white streak on unglazed porcelain plates.


When will palmer appear in Pokemon?

You need to beat a 21 streak and then 50 streak and a 100 streak.


Why must have 4 part in streaking technique?

so that you can get isolated colonies in the last streak . . . As you streak contineously you inoculum quantity decreases . . there by when you reach the end of last streak you get separate and isolated colonies . .


Why do streak plates need to be dry before inoculated?

If by inoculated you mean used, here is my answer if that is true; streak plates need to be dry because the powder left behind may react and change color to whatever that liquid is on the streak plate.


What will happen if the streak plate were incubated a month?

The streak lines would show a lot of colonies.


Why are streak plates used to test minerails?

Scratching a mineral across a streak plate will result in a streak which represents the true color of a mineral without impurities or inclusions that can influence a particular specimen's color. The mineral quartz can appear in a wide variety of colors, but the streak of any colored quartz specimen will still be white. The 'streak' color is one characteristic of a mineral which will aid in its identification.


How does the contribution of growth on a pour plate different from that on a steak plate?

Colonies growing on a pour plate have slightly less avalible oxygen and are confined by the gel matrix so they tend to grow smaller than those on a pour plate. Streak plates are use to isolate single colonies, pour plates are used to enumerate batceria.


What are streak plates used for?

Streaking is used in microbiology to isolate a strain from a species of bacteria, so that this sample can be grown on a new culture.. Streak plates are used in this process to great effect.


How you differentiate between Transformed bacterial colonies and satellite colonies?

Re-streak the center of the 'star' colony (transformed surrounded by satellites) on a plate contains the antibiotic, typically ampicillin. The colonies in the tertiary streak will most likely be the transformants. If you want to be quite sure, pick a single colony from the tertiary streak and re-streak again on a plate containing the antibiotic.