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Which proteolytic enzyme induces lysis of fibrin during fibrinolysis?
Plasmin is the proteolytic enzyme that induces the lysis of fibrin during fibrinolysis. Plasmin breaks down fibrin into soluble fragments, which helps dissolve blood clots.
Why is the secondary antibody used in an ELISA test conjugated with an enzyme?
The secondary antibody in an ELISA test is conjugated with an enzyme to amplify the signal produced when the antibody binds to the target antigen. This enzyme-substrate reaction generates a detectable signal that indicates the presence of the antigen, which allows for more sensitive and accurate detection in the ELISA assay.
What are the differences between indirect ELISA and sandwich ELISA?
Indirect ELISA and sandwich ELISA are two types of enzyme-linked immunosorbent assays used in laboratory testing. In indirect ELISA, the antigen is immobilized on the surface, and a primary antibody binds to the antigen. Then, a secondary antibody linked to an enzyme is added to detect the primary antibody. In sandwich ELISA, the antigen is captured by a primary antibody that is immobilized on the surface. A second antibody linked to an enzyme is then added to bind to a different epitope on the antigen, forming a "sandwich" complex. The main difference between the two methods is the way in which the antibodies are used to detect the antigen. In indirect ELISA, the primary antibody is detected by a secondary antibody, while in sandwich ELISA, the antigen is "sandwiched" between two antibodies for detection.
Does o blood type have a antigens?
Has no antigen in many textbooks it will state "no A-antigen and no B-antigen"(which imply the possibility of some other antigen) and some will even say, "no antigen" (which is true; antigens are things that attach to antigen binding sites, thus, if it does not fit any antigen binding sites, it is technically not a antigen but merely a "enzyme/protein") but this is just to reduce unnecessary and irrelevant information; they are only concerned about A-antibody, B-antibody, A-antigen, and B-antigen. Nonetheless, know that there are in fact antigens on o blood cells, they are just inactive. My guess is, N acetyl glactosamine on A antigen and Galactose on B antigens are Epitopes (: a small specific regions on antigens that are bound by the antigen receptors on lymphocytes and by secreted antibodies.) Antigens without epitopes will not be detected by antigen binding sites.
In the stomach the protein is digested by?
Protein digestion in the stomach is initiated by the enzyme pepsin, which breaks down proteins into smaller peptides. Pepsin is activated by the acidic environment of the stomach, specifically hydrochloric acid. The breakdown of proteins into peptides is essential for their absorption and utilization by the body.
What happens to an enzyme when it is destroyed?
When an enzyme is destroyed, its structure is altered by factors such as high temperature or extreme pH, resulting in loss of its catalytic activity. Once destroyed, an enzyme cannot perform its biological function, leading to impaired biochemical reactions in the cell or organism. The destroyed enzyme is typically broken down into its component amino acids by proteolytic enzymes in the body.
Which proteolytic enzyme induces lysis of fibrin during fibrinolysis?
Plasmin is the proteolytic enzyme that induces the lysis of fibrin during fibrinolysis. Plasmin breaks down fibrin into soluble fragments, which helps dissolve blood clots.
What is least likely to be a mechanism for enzyme regulation?
Change in enzyme concentration through gene expression.
Location and function of pepsin?
pepsin is a proteolytic enzyme present in the gastric glands.it hydrolyses proteins into peptones.
In indirect elisa the enzyme liked antibody will attach to?
In an indirect ELISA, the enzyme-linked antibody attaches to the target antigen that has been immobilized on the microplate. This allows for the detection of the antigen through the enzyme's activity, which produces a signal that indicates the presence of the target antigen in the sample.
Does proteolytic enzymes cause denature of proteins?
Yes, proteolytic enzymes break down proteins by cleaving peptide bonds. This process may result in protein denaturation, especially if the enzyme cleaves at specific sites that disrupt the protein's structure and function.
What will happen if casein is destroyed?
casein is an enzyme destroyed protein nature.
What is a vasodilator that can be inactivated by a proteolytic enzyme?
Nitric oxide is a vasodilator that can be inactivated by proteolytic enzymes such as superoxide dismutase or hemoglobin. These enzymes can break down nitric oxide, reducing its vasodilatory effects.
What is the Enzyme diagnostic?
Diagnostic Antigen is can be used to to detect serum antibodies. creative-biomart.com/list_3.htm
Which enzyme is destroyed by strong acids?
Pepsin is an enzyme in the stomach that is destroyed by strong acids. Pepsin works best at an acidic pH, but too strong of an acid can denature and deactivate the enzyme.
Why is the secondary antibody used in an ELISA test conjugated with an enzyme?
The secondary antibody in an ELISA test is conjugated with an enzyme to amplify the signal produced when the antibody binds to the target antigen. This enzyme-substrate reaction generates a detectable signal that indicates the presence of the antigen, which allows for more sensitive and accurate detection in the ELISA assay.
What are the differences between indirect ELISA and sandwich ELISA?
Indirect ELISA and sandwich ELISA are two types of enzyme-linked immunosorbent assays used in laboratory testing. In indirect ELISA, the antigen is immobilized on the surface, and a primary antibody binds to the antigen. Then, a secondary antibody linked to an enzyme is added to detect the primary antibody. In sandwich ELISA, the antigen is captured by a primary antibody that is immobilized on the surface. A second antibody linked to an enzyme is then added to bind to a different epitope on the antigen, forming a "sandwich" complex. The main difference between the two methods is the way in which the antibodies are used to detect the antigen. In indirect ELISA, the primary antibody is detected by a secondary antibody, while in sandwich ELISA, the antigen is "sandwiched" between two antibodies for detection.
