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Why most of the gram positive bacteria cannot grow on MacConkey Agar?
gram positive bacteria cannot grow due to the presence of bile salts and crystal violet in maConkey media.
What type of bacteria grow on EMB?
Gram Negative Bacilli
What gram positive bacteria will grow on macconkey medium?
Scanning about the microbiologic community, a few references to Gram pos bacteria growing on MacConkey's showed up. One reports a Gram positive rod of unspecified species (see links). Another article says some forms of Enterococcus and Staphylococcus can grow in spite of the bile salts that make this medium so antagonistic to Gram positives. But just as many articles opine that, if this happens, the medium may not have been properly prepared.
Can Serratia grow on Hektoen Enteric Agar?
Yes. Serratia is a Gram negative rod from the Enterobacteriaceae family. The Hektoen Enteric agar allows the growth of Gram negative bacteria while inhibiting growth of Gram positive bacteria.
Can enterobacter aerogenes grow on mannitol salt agar?
the bacteria dont ferment in MSA, there is no growth on the plat
Crystal violet in the MacConkey agar?
MacConkey's agar refers to a culture medium that is designed to selectively grow Gram-negative bacteria. It contains crystal violet dye, which inhibits certain Gram-positive bacteria.
Does micrococcus luteus grow on macconkey agar?
MacConkey agar is designed to grow Gram-negative bacteria and also contains crystal violet dye which inhibits the growth of Gram-positive bacteria. Since S. aureus is Gram-positive it should not grow on MacConkey agar. No. MacConkey agar inhibits MOST of the Gram-positive bacteria. There are some Gram-positive bacteria that can tolerate bile-salt are able to grow in MacConkey agar. Enterococcus and some species of Staphylococcus are able to grow in MacConkey. (lack citation though)
Macconkey agar is a selective medium and it is also a defined or a undefined medium so why is that a formulation desirable?
This type of agar is designed to grow gram-negative bacteria. It will not grow gram-positive bacteria due to a dye in the formula of the agar.
Why most of the gram positive bacteria cannot grow on MacConkey Agar?
gram positive bacteria cannot grow due to the presence of bile salts and crystal violet in maConkey media.
What selective growth media is best suited for isolation of coliform bacteria?
MacConkey's agar is a selective growth media that is best suited for isolation of coliform bacteria. This bacterial is red and is gram negative. The agar plate inhibits the growth of gram positive organisms and allows gram negative organisms to grow.
Which gram positive bacteria grows on macconkey and EMB?
Gram + bacteria do NOT grow on EMB agar, EMB agar inhibits their growth, and is selective for Gram -
Can treatment with Transpeptidase eliminate gram positive bacteria from a mixture of gram positive bacteria and gram negative bacteria?
No. A treatment of ethylenediaminetetraacetic acid (EDTA) and the enzyme, lysozyme, will cause gram negative bacteria to lyse. Some gram positive bacteria can also be lysed by doing this. The EDTA stabilizes the solution and allows lysozyme to cut the bonds between the carbohydrates that make up the cell wall wall in bacteria. This will lyse the cell and cause the contents of the cell to be spilled out. This technique is primarily used for DNA assays. A good way to isolate either gram positive or gram negative is by using different types of agar. The MacConkey agarmight be the most useful for what you want to do. It will only allow gram negative to grow, isolating just the gram negatives only.
Why use Azide blood agar MacConkey agar and Nutrient agar plates for identification of microorganisms?
Azide blood agar base contains sodium azide which has been proved to have a bacteriostatic effect on Gram-negative bacteria, thus this medium is used for the isolation of streptococci and staphylococci in clinical specimens, water, foods, etc. MacConkey agar is designed to grow Gram-negative bacteria and differentiate them for lactose fermentation. Nutrient agar is used for the routine cultivation of non-fastidious bacteria.
What type of bacteria grow on EMB?
Gram Negative Bacilli
What gram positive bacteria will grow on macconkey medium?
Scanning about the microbiologic community, a few references to Gram pos bacteria growing on MacConkey's showed up. One reports a Gram positive rod of unspecified species (see links). Another article says some forms of Enterococcus and Staphylococcus can grow in spite of the bile salts that make this medium so antagonistic to Gram positives. But just as many articles opine that, if this happens, the medium may not have been properly prepared.
Can Serratia grow on Hektoen Enteric Agar?
Yes. Serratia is a Gram negative rod from the Enterobacteriaceae family. The Hektoen Enteric agar allows the growth of Gram negative bacteria while inhibiting growth of Gram positive bacteria.
What are the EMB test results for Staphylococcus epidermidis?
The Eosine Methylene Blue test is selective for only gram-negative bacteria. It is possible for gram-positive bacteria to grow, but it would barely grow. Staphylococcus are gram-positive spherical bacteria. So it would not grow on the Eosine methylene Blue Agar.
