gram positive bacteria cannot grow due to the presence of bile salts and crystal violet in maConkey media.
The conclusion drawn if no growth appeared on MacConkey agar and EMB agar after inoculation of the media and an incubation period could be the bacteria used was possibly a Gram positive non-enteric sample.
ecoli is a lactose metabolizer, can live on macconkey while psuedomoas cannot.
to differentiate between the lactose fermented bacteria or non- lactose fermented bacteria
MacConkey Agar plate
yes
MacConkey agar is designed to grow Gram-negative bacteria and also contains crystal violet dye which inhibits the growth of Gram-positive bacteria. Since S. aureus is Gram-positive it should not grow on MacConkey agar. No. MacConkey agar inhibits MOST of the Gram-positive bacteria. There are some Gram-positive bacteria that can tolerate bile-salt are able to grow in MacConkey agar. Enterococcus and some species of Staphylococcus are able to grow in MacConkey. (lack citation though)
it inhibits the growth of Gram positive bacteria
MacConkey's agar refers to a culture medium that is designed to selectively grow Gram-negative bacteria. It contains crystal violet dye, which inhibits certain Gram-positive bacteria.
Macconkey agar consists of agar, lactose, peptone, neutral red dye, crystal violet dye & bile salts. Bile salts inhibit growth of Gram positive bacteria & allow growth of Gram negative bacteria only. It differentiates lactose fermentating Gram negative bacteria from non lactose fermentating Gram negative bacteria.
This type of agar is designed to grow gram-negative bacteria. It will not grow gram-positive bacteria due to a dye in the formula of the agar.
The conclusion drawn if no growth appeared on MacConkey agar and EMB agar after inoculation of the media and an incubation period could be the bacteria used was possibly a Gram positive non-enteric sample.
The conclusion drawn if no growth appeared on MacConkey agar and EMB agar after inoculation of the media and an incubation period could be the bacteria used was possibly a Gram positive non-enteric sample.
Gram + bacteria do NOT grow on EMB agar, EMB agar inhibits their growth, and is selective for Gram -
types macconkey agar
Lac+ bacteria is acid base color indicator that lowers the pH of the MacConkey agar. The MacConkey agar contains neutral red dye, lactose, peptone, and crystal violet dye.
ecoli is a lactose metabolizer, can live on macconkey while psuedomoas cannot.
to differentiate between the lactose fermented bacteria or non- lactose fermented bacteria