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gram positive bacteria cannot grow due to the presence of bile salts and crystal violet in maConkey media.

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Q: Why most of the gram positive bacteria cannot grow on MacConkey Agar?
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Does micrococcus luteus grow on macconkey agar?

MacConkey agar is designed to grow Gram-negative bacteria and also contains crystal violet dye which inhibits the growth of Gram-positive bacteria. Since S. aureus is Gram-positive it should not grow on MacConkey agar. No. MacConkey agar inhibits MOST of the Gram-positive bacteria. There are some Gram-positive bacteria that can tolerate bile-salt are able to grow in MacConkey agar. Enterococcus and some species of Staphylococcus are able to grow in MacConkey. (lack citation though)


Role of bile salt in macconkey agar?

it inhibits the growth of Gram positive bacteria


Crystal violet in the MacConkey agar?

MacConkey's agar refers to a culture medium that is designed to selectively grow Gram-negative bacteria. It contains crystal violet dye, which inhibits certain Gram-positive bacteria.


What is macconkey agar made out of?

Macconkey agar consists of agar, lactose, peptone, neutral red dye, crystal violet dye & bile salts. Bile salts inhibit growth of Gram positive bacteria & allow growth of Gram negative bacteria only. It differentiates lactose fermentating Gram negative bacteria from non lactose fermentating Gram negative bacteria.


Macconkey agar is a selective medium and it is also a defined or a undefined medium so why is that a formulation desirable?

This type of agar is designed to grow gram-negative bacteria. It will not grow gram-positive bacteria due to a dye in the formula of the agar.


What conclusions may be drawn if no growth appeared on MacConkey agar and emb agar after inoculation of the media and an incubation period?

The conclusion drawn if no growth appeared on MacConkey agar and EMB agar after inoculation of the media and an incubation period could be the bacteria used was possibly a Gram positive non-enteric sample.


What conclusion may be drawn if no growth appeared on MacConkey agar and EMB agar after inoculation of the media and an incubation period?

The conclusion drawn if no growth appeared on MacConkey agar and EMB agar after inoculation of the media and an incubation period could be the bacteria used was possibly a Gram positive non-enteric sample.


Which gram positive bacteria grows on macconkey and EMB?

Gram + bacteria do NOT grow on EMB agar, EMB agar inhibits their growth, and is selective for Gram -


What type of medium is macconkey agar?

types macconkey agar


What is the name of the acide base color indicator found in MacConkey agar?

Lac+ bacteria is acid base color indicator that lowers the pH of the MacConkey agar. The MacConkey agar contains neutral red dye, lactose, peptone, and crystal violet dye.


What differences between Ecoli and pseudomonas sp on MacConkey agar?

ecoli is a lactose metabolizer, can live on macconkey while psuedomoas cannot.


What is the purpose of lactose in the macconkey agar?

to differentiate between the lactose fermented bacteria or non- lactose fermented bacteria