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Which medium is most difficult to prepare without contamination?
Wiki User
∙ 9y ago
I think none of them is difficult to prepare without contamination as if you prepare any media in any of tube or plate or in flask just take care of all the important thinghs like proper autoclaving of media proper sterlization of instruments and proper handiling in pouring i.e. pour media in clean LAF, wash hands with antimicrobial soap or foam and pour media near the flame. But if still says then it will be easy for preparing nutrient agar tubes as u can pour the media in them and then you can sterlize them in autoclave after plugging the cotton plug, and that will be without any contamination same u can also do with nutrient broth in eirenmyere flasks
Wiki User
∙ 15y ago
Wiki User
∙ 9y agoThe medium that was most difficult to prepare without contamination was the quadrant streak method. It is important to use a medium soon after it has been prepared so that oxygen doesn't diffuse and ruin results.
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Inoculating loop is used to inoculate microbial colony or sample on culture medium and to avoid the undesired microbial cells or to avoid contamination flaming of inoculating loop is necessary it is also called as incerination.
Why is blood agar not useful a primary isolation medium?
Agar should be sterile and have limited nutrition for a primary isolation. Agar derived from blood would most likely encourage the growth of a wide variety of microorganisms, making it difficult to isolate the population you are interested in
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A basal medium is a growth medium which incorporates a genetic material such as yeast extract of unknown precise composition.
What algae is used to prepare a medium for bacterial culture?
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Why is undefined medium desirable?
It is desirable because it is easier to prepare.
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Light can travel with and without a medium.
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Red Algae ?
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It is recommended to prepare ferrous ammonium sulphate solution in acidic medium because iron when combined with water gives the precipitates of oxides of Iron.
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They do not need a medium.
How can you detect that you have prepared and transferred a medium without any contamination?
It's hard to tell without a microscope. The best way to do this is just to follow sterile techniques. Be certain that you have sterilized everything at every step, and work slowly and carefully. Otherwise, you'll know when the bacteria or fungi start growing!
What can travel through space without a(n) medium?
Electromagnetic waves can travel through space without a medium.
What can be transmitted with or without a medium?
light
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