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Which of the three main steps of pcr is responsible for the specificity in the small piece of DNA that gets amplified by replication?
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∙ 14y ago
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∙ 14y ago
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Is replication the storing of a piece of data in more than one place?
true
What is an end replication problem?
The two strands of a DNA molecule are antiparallel to one another (the backbone of one strand runs from 5'-3' while the complimentary strand runs 3'-5'). Unfortunately, DNA polymerase, the enzyme responsible for replicating DNA, can only make DNA in a 5'-3' direction (and read DNA in the 3'-5' direction). Also, it needs a "primer" to give it a place to bind and start replication. So this creates a problem when synthesizing the 3'-5' stand because your enzyme will only synthesize 5'-3'. During replication this is solved by synthesizing small pieces of DNA ahead of the replication fork on the 5'-3' mother strand. Thus we have one daughter strand which is synthesized as a continuous piece of DNA (called the leading strand) and one daughter strand which is synthesized in small, discontinuous pieces (called the lagging strand). However, at the extreme end of the DNA, we run into another problem. The leading stand can be made to the very end, but the lagging strand cannot because you need the RNA primer upstream to begin each piece of the lagging strand DNA but at the end of the DNA there is nothing for this piece to attach to. Thus, the last section of the lagging strand cannot be synthesized and after several rounds of DNA replication, the DNA molecule gets smaller and smaller. This is "the end of replication problem" and it is solved by putting a DNA cap on the ends of DNA called a telomere which does not code for any protein, thus when this information is lost it does not have severe consequences for the cell.
What is a primer in biology?
Primer is a piece of mRNA that replicates first in the DNA replication. The creation of primer is facilitated by the enzyme called PRIMASE which facilitates DNA replication along with HELICASE, an enzyme that breaks Hydrogen bonds to help unwind the DNA.
What is function of midpiece of sperm cell?
Middle piece is formed of mitochondiral spiral , nebenkern around the proximal part of axoneme. The mitochondria are the carriers of the oxidative enzymes and the enzymes which are responsible for oxidative phosphorylation. So the middle piece is the power house of a sperm
Why is DNA replication called semiconservative?
DNA replication of one helix of DNA results in two identical helices. If the original DNA helix is calledthe "parental" DNA, the two resulting helices can be called "daughter" helices. Each of these two daughter helices is a nearly exact copy of the parental helix (it is not 100% the same due to mutations).DNA creates "daughters" by using the parental strands of DNA as a template or guide. Each newly synthesized strand of DNA (daughter strand) is made by the addition of a nucleotide that is complementary to the parent strand of DNA. In this way, DNA replication is semi-conservative, meaning that one parent strand is always passed on to the daughter helix of DNA.
Is replication the storing of a piece of data in more than one place?
true
What is an example of word choice?
Word choice can make a big difference for the tone of a writer's essay. It is based on context, diction, and specificity of the piece.
How do you think the masks were able to amplify the actor's voice in greek theatre.?
it was a metal piece in the mouth of the mask that amplified th voice
What is the piece of a computer responsible for providing electricity to all of the internal parts?
The power supply.
You signed a contract stating that you were personally responsible for the debt even though you are a llc are you responsible?
Of course you are responsible. you are the one that chise to sign that piece of paper. That's why you have to fully read things before you ever sign your name to anything. Of course you are responsible. you are the one that chise to sign that piece of paper. That's why you have to fully read things before you ever sign your name to anything.
What is an end replication problem?
The two strands of a DNA molecule are antiparallel to one another (the backbone of one strand runs from 5'-3' while the complimentary strand runs 3'-5'). Unfortunately, DNA polymerase, the enzyme responsible for replicating DNA, can only make DNA in a 5'-3' direction (and read DNA in the 3'-5' direction). Also, it needs a "primer" to give it a place to bind and start replication. So this creates a problem when synthesizing the 3'-5' stand because your enzyme will only synthesize 5'-3'. During replication this is solved by synthesizing small pieces of DNA ahead of the replication fork on the 5'-3' mother strand. Thus we have one daughter strand which is synthesized as a continuous piece of DNA (called the leading strand) and one daughter strand which is synthesized in small, discontinuous pieces (called the lagging strand). However, at the extreme end of the DNA, we run into another problem. The leading stand can be made to the very end, but the lagging strand cannot because you need the RNA primer upstream to begin each piece of the lagging strand DNA but at the end of the DNA there is nothing for this piece to attach to. Thus, the last section of the lagging strand cannot be synthesized and after several rounds of DNA replication, the DNA molecule gets smaller and smaller. This is "the end of replication problem" and it is solved by putting a DNA cap on the ends of DNA called a telomere which does not code for any protein, thus when this information is lost it does not have severe consequences for the cell.
Is the red-shift responsible for the big bang theory?
No, it's the other way round. The Big Bang is responsible for the redshift.
What is the difference between replication of DNA and amplifcation of DNA in PCR?
DNA cloning is where you take a piece of DNA and put it in a host cell so that every time the host cell replicates, its daughter cells will have that exact copy of DNA. DNA amplification is just taking a piece of DNA and making copies of it, like in the process of PCR. it is not inside a host cell. another way to think of it: you can amplify a gene--make a bunch of copies of it, and then clone it (by putting it in a cell and once that cell replicates each daughter cell has a copy of that DNA). you don't need to amplify anymore in cloning, you already did that before.
Was the Treaty of Versailles responsible for World War 2?
there were various other reasons than just a piece of paper.
What is name of the artist primarily responsible for Sagrada Familia Cathedral?
It is a master piece made by Antoni Gadui i cornet.
When you eat a piece of bread you consume carbohydrates which part of the the cell is responsible for breaking down carbohydrate for use by the body?
Lysosomes
WHEN YOU EAT A PIECE OF BREAD YOU CONSUME CARBOHYDRATES. WHICH PART OF THE CELL IS RESPONSIBLE FOR BREAKING DOWN A CARBOHYDRATES FOR USE BY THE BODY?
Lysosomes
