0
What else can I help you with?
What are the properties that differentiate the spore from the vegetative cell?
In the spore stain malachite green is used as a primary stain. This is driven into the cell by heat because of the impermeability of the spore. The stain is allowed to sit for 30 mins to make sure it gets in to the endospores.The stain is then washed and counterstained with safranin red. The endospores retain the green colour from malachite green and of course appear green under the microscope. Whereas the vegetative cells will appear red.
What happens if you don't heat fix a smear prior to staining of a bacterial slide?
If a bacterial smear is not heat fixed prior to staining, the bacteria may not adhere well to the slide and can wash away during the staining process. Heat fixing helps to kill the bacteria, firmly attach them to the slide, and improve the uptake of stain, resulting in better staining results. Without heat fixing, the bacteria may not stain properly or may not be visible at all under the microscope.
What would you expect from a gram stain done on a slide that was heated too hot during the heat-fixed smear?
A Gram stain refers to a staining technique for the identification of bacteria. A Gram stain done on a slide that was heated too hot during the heat-fixed smear will destroy the cell wall of the bacteria.
What is the riquirement of spore staining?
I can remember from my last experiment that the major stainused is Malachite green.It has the capability of sticking hard to spore's membranes.1st you prepare a wetmount,you airdry or dry it with a bunsen's flame.Then when it got completely dried,stain with malachite green.Then place the slide on top of boiling h20 in a beaker and allow to stand for 30mins. But always add some few drops of the stain so that the slide won't get dry and give false +ve results. Then after 30mins,put a drop of immersion oil and view under *40 objective.You'll view spores surrounded with a green sheath,which would your expected result. Good luck,it's been cedrik storm,garvey02@yahoo.com
Why gram staining procedure cannot stain endospores?
Endospores have a unique structure with thick layers of protein and peptidoglycan that resist the staining process used in Gram staining. The dye used in Gram staining is unable to penetrate these layers, resulting in endospores not taking up the stain. Specialized staining techniques, such as the Schaeffer-Fulton method, are required to visualize endospores.
What mordant is used in spore staining?
Heat is the mordant used in the spore stain, it fixes the primary stain.
What would the results be if you used the TB decolorizer during the spore stain?
Depends if heat is used
What is the purpose of staining the smear in malachite green during spore staining?
the purpose of boiling of smear in malachite green is to forces a stain to penetrate the endospore wall, it is necessary to heat the slide and the stain to prod the wall to allow the stain to enter.
In spore staining what would happen it you used safarin as the primary stain and malachite green as the counterstain?
The spore would appear to be red as the safranin is heat driven into the many layers of the spore, however, as Malachite green has a weak affinity and is water soluble, it will not likely bind to the spore wall or the cell wall. You might have traces of green on the slide if any, but it will be very little. Your vegetative cells will be pink as well.
Why is it essential to apply heat during endospore staining?
Applying heat during endospore staining helps in the penetration of the primary stain, usually malachite green, into the endospore wall. Heat acts as a mordant that allows the stain to bind more effectively to the endospore, enhancing its visibility under the microscope. This technique improves the contrast between the endospore and the rest of the cell, aiding in their identification and study.
What does a positive result for spore stain indicate?
A positive result for spore stain indicates the presence of bacterial endospores. Endospores are a dormant, highly resistant form of some bacteria that allows them to survive harsh conditions such as heat, desiccation, and chemical disinfectants. Detection of spores can be important in identifying certain pathogenic or environmental bacteria.
How do you know when to do a spore stain?
endospores can't be stained by ordinary methods, such as simple and gram staining, because the stain can't penetrate the endospore's wall, therefore you must heat the stain to help it penetrate the wall
What are the properties that differentiate the spore from the vegetative cell?
In the spore stain malachite green is used as a primary stain. This is driven into the cell by heat because of the impermeability of the spore. The stain is allowed to sit for 30 mins to make sure it gets in to the endospores.The stain is then washed and counterstained with safranin red. The endospores retain the green colour from malachite green and of course appear green under the microscope. Whereas the vegetative cells will appear red.
What happens if you don't heat fix a smear prior to staining of a bacterial slide?
If a bacterial smear is not heat fixed prior to staining, the bacteria may not adhere well to the slide and can wash away during the staining process. Heat fixing helps to kill the bacteria, firmly attach them to the slide, and improve the uptake of stain, resulting in better staining results. Without heat fixing, the bacteria may not stain properly or may not be visible at all under the microscope.
What would you expect from a gram stain done on a slide that was heated too hot during the heat-fixed smear?
A Gram stain refers to a staining technique for the identification of bacteria. A Gram stain done on a slide that was heated too hot during the heat-fixed smear will destroy the cell wall of the bacteria.
What is the riquirement of spore staining?
I can remember from my last experiment that the major stainused is Malachite green.It has the capability of sticking hard to spore's membranes.1st you prepare a wetmount,you airdry or dry it with a bunsen's flame.Then when it got completely dried,stain with malachite green.Then place the slide on top of boiling h20 in a beaker and allow to stand for 30mins. But always add some few drops of the stain so that the slide won't get dry and give false +ve results. Then after 30mins,put a drop of immersion oil and view under *40 objective.You'll view spores surrounded with a green sheath,which would your expected result. Good luck,it's been cedrik storm,garvey02@yahoo.com
Why do you heat the slide during the root tip squash experiment?
The heating allows the stain to intensify, therefore allowing the chromosomes to be seen more clearly
