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What color is moraxella catarrhalis after the decolorizer is used?
Moraxella catarrhalis is a Gram negative bacterium. Thus, after the decolorizer is used, its thin cell wall won't retain the primary stain. The stain is washed away, and Moraxella catarrhalis is colorless.
What is the decolorizer used in the capsule stain?
The decolorizer used in the capsule stain is a dilute solution of 20% copper sulfate. It is used to remove the purple crystal violet stain from the background and bacterial cells, leaving only the capsule stained.
How does culture age affect results of a spore stain?
The age of the culture used for a spore stain can impact the results by affecting the viability and sporulation of the organism. A young culture with actively growing cells is more likely to produce good spore stain results, while an older culture with decreased viability and sporulation may lead to unreliable staining outcomes. It is generally recommended to use a fresh culture for spore staining to obtain accurate and reliable results.
What mordant is used in spore staining?
Heat is the mordant used in the spore stain, it fixes the primary stain.
How do you make decolorizer for gram stain?
It is an alchoholic compound i.e. 95% ethanol.
Assume that during the performance of this exercise you made several errors in your spore staining procedure?
Assume that during the performance of this exercise you made several errors in your spore-staining procedure. In each of the following cases indicate how your microscopic observations would differ from those observed when the slides were prepared correctly . A. you used acid-alcohol as the decolorizing agent . B. you used safranin as the primary stain and malachite green as the counterstain C. you did not apply heat during the application of the primary stain · A. Normally tap water is used as the decolorizing agent to wash off excess stain. When you use acid-alcohol, it decolorizes the cells and the stain is removed. · B.When you use safranin as the primary stain and malachite green as the secondary stain, the cells will stain green and the spores will stain red. · C.When heat is not applied during the application of the primary stain, the spores are not stained and they appear colorless.
What does a negative result for a spore stain indicate about the organism?
A negative result for a spore stain indicates that the organism does not form endospores. Endospore formation is a survival mechanism for some bacteria to withstand harsh environments, so a negative result suggests that the organism may be more susceptible to environmental stressors.
What is the negative spore stain color?
The negative spore stain color is pink or red, indicating that the spores are colorless or only weakly stained compared to the rest of the cell. This is in contrast to the positive spore stain, where the spores appear green due to the malachite green staining.
What is the purpose of staining the smear in malachite green during spore staining?
the purpose of boiling of smear in malachite green is to forces a stain to penetrate the endospore wall, it is necessary to heat the slide and the stain to prod the wall to allow the stain to enter.
What color do bacterial endospores stain in the Schaeffer-Fulton spore stain?
Bacterial endospores stain green in the Schaeffer-Fulton spore stain due to the malachite green dye used. This technique is commonly used to visualize endospores in bacteria.
What does the decolorizer do in the process of gram staining?
The decolorizer in the process of gram staining removes the purple stain from bacteria that do not retain it, allowing them to be stained with a contrasting color. This step helps differentiate between gram-positive and gram-negative bacteria based on their cell wall composition.
What is the decolorizing agent in the acid fast stain?
The decolorizing agent in the acid fast stain is acid alcohol. The decolorizing agent in the gram stain is ethanol.
