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What color is moraxella catarrhalis after the decolorizer is used?
Wiki User
∙ 13y ago
Moraxella catarrhalis is a Gram negative bacterium. Thus, after the decolorizer is used, its thin cell wall won't retain the primary stain. The stain is washed away, and Moraxella catarrhalis is colorless.
Wiki User
∙ 11y ago
Wiki User
∙ 13y agoPink
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What is the purpose of alcohol used in the gram stain?
The alcohol is a decolorizer. In gram negative organisms, the small amount of peptidoglycan can't hold onto the crystal violet in the presence of the alcohol and so becomes decolorized. The gram positive organisms have a much thicker peptidoglycan layer, and so the crystal violet stays in even with washing by alcohol.
What can be used for a simple stain procedure?
Perhaps Gram Staining? Steps are as follows: 1. Crystal Violet, 2. Iodine, 3. Decolorizer, 4. Safrinin
How can you remove crystal violet in a Gram Stain?
You won't be able to positively distinguish gram negative from gram positive organisms. Crystal violet is used first so that gram positive organisms will take up the dye and this color is not affected by safrinin later on. Gram negative organisms will lose the purple coloring during the decolorizer step, therefore need to be counterstained so that you can view the organisms.
Purpose of decolorizing agent?
The decolorizer, usually acetone or alcohol, is used to wash the Crystal Violet stain from the Gram Negative cells. From this point Safranin stain is used to stain the Gram Negative cells. The final color for Gram Negative will be a Red/Pink color.
What would be the result of the gram stain if acid alcohol is used as a decolorizer rather than the gram decolorizer?
alcohol will remove the lipid layer of the G- cells and the outer membrane. Edit: Ethyl alcohol is used as a decolorization step. Without using ethyl alcohol the gram-negative cells will still remain colored. H2O may dilute the cells or wash them off the slide.
How do you recognize diplococci?
A diplococcus is a round bacterium that typically occurs in pairs of two joined cells. Examples are Streptococcus pneumoniae, Moraxella catarrhalis, Neisseria gonorrhoeae and Neisseria meningitidis. Of these, all are Gram-negative except for Streptococcus Pneumoniae.Its name comes from diplo, meaning double, and coccus, meaning berry.In former times, a bacterial genusDiplococcus was recognized, but it is not used anymore.
What would the results be if you used the TB decolorizer during the spore stain?
Depends if heat is used
Why is decolorization necessary when performing a gram stain?
During the procedures of a gram stain, decolorization is necessary to remove any stain or color from the gram negative cells. When a dye is used to stain gram positive cells, both gram positive and gram negative cells retain color. Mordant is used to bind the original stain to gram positive cells so when decolorizer is used they retain color. After the mordant has been used a decolorizer is used to wash away colo in gram negative cells. Counterstains are used to stain gram negative cells to better visualize contrasting cells. An example of a decolorizer that works well is ethanol.
Which bacteria does amoxicillin kill?
Amoxicillin is an antibiotic medication that is used to treat a variety of bacterial infections. It is a type of penicillin meaning it works by inhibiting the growth of bacteria. Amoxicillin is effective against many different types of bacteria including: Streptococcus pneumoniae Haemophilus influenzae Moraxella catarrhalis Staphylococcus aureus Escherichia coliAmoxicillin is also effective against some types of streptococcus bacteria including Streptococcus pyogenes and Streptococcus agalactiae. Other bacteria that can be killed by amoxicillin include Listeria monocytogenes Enterococcus faecalis and Proteus mirabilis.
What is the purpose of alcohol used in the gram stain?
The alcohol is a decolorizer. In gram negative organisms, the small amount of peptidoglycan can't hold onto the crystal violet in the presence of the alcohol and so becomes decolorized. The gram positive organisms have a much thicker peptidoglycan layer, and so the crystal violet stays in even with washing by alcohol.
What can be used for a simple stain procedure?
Perhaps Gram Staining? Steps are as follows: 1. Crystal Violet, 2. Iodine, 3. Decolorizer, 4. Safrinin
What are two reasons why gram-positive cells may appear pink after the completion of the gram-stain?
If they were old and the cell membrane had degraded, or if you used too much decolorizer.
How can you remove crystal violet in a Gram Stain?
You won't be able to positively distinguish gram negative from gram positive organisms. Crystal violet is used first so that gram positive organisms will take up the dye and this color is not affected by safrinin later on. Gram negative organisms will lose the purple coloring during the decolorizer step, therefore need to be counterstained so that you can view the organisms.
What does co-amoxiclav 625 treat?
what the pro blame i tack before meal moxlclav tablate
Purpose of decolorizing agent?
The decolorizer, usually acetone or alcohol, is used to wash the Crystal Violet stain from the Gram Negative cells. From this point Safranin stain is used to stain the Gram Negative cells. The final color for Gram Negative will be a Red/Pink color.
What is the difference between ZN stain and modified ZN stain?
in Zn we use 3% decolorizer, while in modified 1% h2so4 is used, modified is used for paracite like Cryptosporidium, cyclosporidium and bacterias like nocaddia and actinomyces. also in modified heating isn't done.
What would be the result of the gram stain if acid alcohol is used as a decolorizer rather than the gram decolorizer?
alcohol will remove the lipid layer of the G- cells and the outer membrane. Edit: Ethyl alcohol is used as a decolorization step. Without using ethyl alcohol the gram-negative cells will still remain colored. H2O may dilute the cells or wash them off the slide.
