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What would be the result of the gram stain if acid alcohol is used as a decolorizer rather than the gram decolorizer?
Wiki User
∙ 11y ago
alcohol will remove the lipid layer of the G- cells and the outer membrane.
Edit: Ethyl alcohol is used as a decolorization step. Without using ethyl alcohol the gram-negative cells will still remain colored. H2O may dilute the cells or wash them off the slide.
Wiki User
∙ 11y ago
Wiki User
∙ 11y agoColor fastness is a characteristic of certain microbes that resist decolorization with acid alcohol. Cells that resist this decolorizing process are known as acid fast organisms, or simply, acid fast. They absorb the new dye
Wiki User
∙ 15y agoSome decolorizing of the sample would take place but not as much as using the acid alcohol. Acid alcohol is stronger because it contains hydrochloric acid.
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What is the purpose of alcohol used in the gram stain?
The alcohol is a decolorizer. In gram negative organisms, the small amount of peptidoglycan can't hold onto the crystal violet in the presence of the alcohol and so becomes decolorized. The gram positive organisms have a much thicker peptidoglycan layer, and so the crystal violet stays in even with washing by alcohol.
What color is moraxella catarrhalis after the decolorizer is used?
Moraxella catarrhalis is a Gram negative bacterium. Thus, after the decolorizer is used, its thin cell wall won't retain the primary stain. The stain is washed away, and Moraxella catarrhalis is colorless.
Purpose of decolorizing agent?
The decolorizer, usually acetone or alcohol, is used to wash the Crystal Violet stain from the Gram Negative cells. From this point Safranin stain is used to stain the Gram Negative cells. The final color for Gram Negative will be a Red/Pink color.
What is the difference between Jensen's version ang hucker's modification in gram staining based on reagents and gram reaction to fungi?
What is modified in Jensen's modification of Gram stain? Jensen's modification: This method involves use to methyl violet as primary stain, iodine and potassium iodide in water as mordant, absolute alcohol as decolorizer and neutral red as counterstain
What can be used for a simple stain procedure?
Perhaps Gram Staining? Steps are as follows: 1. Crystal Violet, 2. Iodine, 3. Decolorizer, 4. Safrinin
How do you make decolorizer for gram stain?
It is an alchoholic compound i.e. 95% ethanol.
What is the decolorizing agent in the acid fast stain?
The decolorizing agent in the acid fast stain is acid alcohol. The decolorizing agent in the gram stain is ethanol.
What is the purpose of alcohol used in the gram stain?
The alcohol is a decolorizer. In gram negative organisms, the small amount of peptidoglycan can't hold onto the crystal violet in the presence of the alcohol and so becomes decolorized. The gram positive organisms have a much thicker peptidoglycan layer, and so the crystal violet stays in even with washing by alcohol.
What color is moraxella catarrhalis after the decolorizer is used?
Moraxella catarrhalis is a Gram negative bacterium. Thus, after the decolorizer is used, its thin cell wall won't retain the primary stain. The stain is washed away, and Moraxella catarrhalis is colorless.
Purpose of decolorizing agent?
The decolorizer, usually acetone or alcohol, is used to wash the Crystal Violet stain from the Gram Negative cells. From this point Safranin stain is used to stain the Gram Negative cells. The final color for Gram Negative will be a Red/Pink color.
What would the results be if you used the TB decolorizer during the spore stain?
Depends if heat is used
What is the difference between Jensen's version ang hucker's modification in gram staining based on reagents and gram reaction to fungi?
What is modified in Jensen's modification of Gram stain? Jensen's modification: This method involves use to methyl violet as primary stain, iodine and potassium iodide in water as mordant, absolute alcohol as decolorizer and neutral red as counterstain
What can be used for a simple stain procedure?
Perhaps Gram Staining? Steps are as follows: 1. Crystal Violet, 2. Iodine, 3. Decolorizer, 4. Safrinin
What are two reasons why gram-positive cells may appear pink after the completion of the gram-stain?
If they were old and the cell membrane had degraded, or if you used too much decolorizer.
Why is decolorization necessary when performing a gram stain?
During the procedures of a gram stain, decolorization is necessary to remove any stain or color from the gram negative cells. When a dye is used to stain gram positive cells, both gram positive and gram negative cells retain color. Mordant is used to bind the original stain to gram positive cells so when decolorizer is used they retain color. After the mordant has been used a decolorizer is used to wash away colo in gram negative cells. Counterstains are used to stain gram negative cells to better visualize contrasting cells. An example of a decolorizer that works well is ethanol.
How can a coke stain be removed from a leather bag?
Rubbing alcohol all over the stain
What is the result of gram stain on human cell?
Human cells have no cell walls, only a plasma membrane, and as a result lack the peptidoglycan layer that gram stains utilize to differentiate between gram negative and gram positive species of bacteria. Therefore, human cells are unable to retain the crystal violet introduced in the first step of the gram stain, and stain negative. (they appear pinkish like gram-negative bacteria)
