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The decolorizing agent in the acid fast stain is acid alcohol. The decolorizing agent in the gram stain is ethanol.
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Why is acid alcohol rather than ethyl alcohol used as a decolorizing agent in the acid fast staining experiment?
Alcohol is a term used for any O-H group that is attached to a carbon. Perhapes the alcohol is found in a Safranin stain. I hope I have this right but if alcohol was used as the decolorizing agent, it may wash out too much stain to get a good view in an oil emersion microscope.
How fast does half a stick of chalk dissolve in water?
In order to dissolve chalk in water, vinegar needs to be added. The vinegar has acid in it that eats away at the chalk to dissolve it.
What happens when you mix a strong acid with a strong base?
When a strong acid and a strong base mix, all acidic protons will react with every basic molecule until one or the other runs out. The curve for a titration of a strong acid with a strong base will change slowly at first, and dramatically when the equivalence point (where the number of moles of acid is equal to the number of moles of base) is reached. The reaction, like all acid-base reactions, is fast.
How fast is a megacycle?
not very fast
which of the following baroque forms follows the pattern fast-slow-fast?
Concerto is the baroque form following the pattern fast-slow-fast.
Why is acid alcohol rather than ethyl alcohol used as a decolorizing agent in the acid fast staining experiment?
Alcohol is a term used for any O-H group that is attached to a carbon. Perhapes the alcohol is found in a Safranin stain. I hope I have this right but if alcohol was used as the decolorizing agent, it may wash out too much stain to get a good view in an oil emersion microscope.
Are endospores acid fast?
No, endospores are not acid fast because they are not cells. Endospores will stain with the acid fast stain and look fuchsia but they are not positive for acid fast.
Is M. tuberculosis acid fast or non-acid fast?
Mycobacterium tuberculosis is an acid fast bacterium. It has a high concentration of mycolic acids in the plasma membrane which prevent its staining by typical Gram stain methods. It must be stained with a procedure containing an acid decolorizing step to best visualize it under the microscope (Ziehl Nielson or Kinyon Methods). It resists decolorization with the acid, which is where the term "Acid Fast" comes from....
What are the name of cold acid fast stain?
modified kinyoun's acid fast stain (cold)
Is mordant used in acid fast stain?
The heat is the mordant for an acid-fast stain
What color is Brilliant green K in Acid fast cell?
Acid-fast bacteria stain pink. Non acid-fast bacteria stain green.
What are the advantages and disadvantages of acid fast stain?
Acid Fast Stains Suck Anus
Which color is common to both the gram stain and the acid-fast stain?
purple
Is the mycobacterium smegmatis positive to acid-fast?
NO, s. epidermidis does not show up positive in an acid fast stain. The acid fast stain is a differential stain that differentiates cells with mycolic acids in their cells walls with those who do not. cells with mycolic acid are therefore acid fast. s epidermidis does not contain this waxy substance in their cell wall so it doesnt fall into this category.
Is a Gram stain an adequate substitute for an acid fast stain?
So few organisms are acid-fast, the acid fast stain is used only when infection by an acid-fast organisms is suspected.
Why harsh treatment is necessary in acid-fast stain?
becausee..
Why is the counter stain for gram stain procedure differ from the counter stain used for acid fast procedure?
Safranin
