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Enzymes are thought to function primarily by stabilizing the transition state of the reaction. By binding the transition state more tightly than either the substrates or the products, the enzyme lowers the energy barrier of the reaction. Thus a transition state analog will bind more tightly to the enzyme than either the substrates or the products, preventing them from binding to the enzyme and reacting.

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What causes smear instead of a clear band in PCR?

A smear in your PCR can be caused by too low annealing temperature. Too much or little magnesium or poor primer design or most likely poorly extracted DNA for your template. Precipitate your template DNA again with 70% ethanol give it a good dry and re-elute and try once more. You can also try diluting your template. If there are PCR inhibitors in your DNA prep then using less template can improve results. If you have plenty of DNA in your prep then a 10 or even 100 fold dilution shouldn't prevent good amplification.


Factor that effect enzyme function?

Factors that can affect enzyme function include temperature, pH levels, substrate concentration, enzyme concentration, and the presence of inhibitors or activators. Changes in any of these factors can alter the activity of enzymes and either increase or decrease their ability to catalyze reactions.


Common plant in Texas?

Pecan tree and a Bluebonnet which is a flower. To find out more about common plants in Texas go online to google.com or the website: 50states.com Good Luck! Find out more!


How do I renew my histology technician license?

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What is the difference between wellness and illness?

The difference between illness and wellness, is that illness is a state experienced by the body when one or more of the control systems loose the ability to maintain homeostatis. On the other half wellness is the promotion of health though preventive measures and the pratice of good health habits.

Related Questions

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What are some common inhibitors?

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Transition words in essay causes and effects?

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