Due to the transparent nature of cells and their organelles we use stain to view them. If we didn't stain them we'd barely be able to see the cell let alone the organelles that reside within it.
to enrich the contrast.
It's not funny to see everything just in white/marble and then try to distinguish one element from the other, is it?
Methylene blue stain is used to stain plant and animal cells.
since you do not heat fix the slide when you use a negative stain the cells do not shrink or become distorted
The gram stain uses a decolorizing product so it is possible to differentiate between the gram and the gram cells. Gram positive cells stain purple in color.
it is a basic dye that will stain the cells. That makes it a positive stain.
There is a certain chemical you can use, but a dark colored food coloring works.
The use of endospore stain is to see specialized cell structures. It can tell if some bacterium cells contain higher resistant spores within vegetative cells.
Methylene blue stain is used to stain plant and animal cells.
a microscope!!
since you do not heat fix the slide when you use a negative stain the cells do not shrink or become distorted
The gram stain uses a decolorizing product so it is possible to differentiate between the gram and the gram cells. Gram positive cells stain purple in color.
it is a basic dye that will stain the cells. That makes it a positive stain.
There is a certain chemical you can use, but a dark colored food coloring works.
methelyn blue
one disadvantage of using iodine to stain cells is that iodine will kill living cells.
Sudan black B stainThis stain distinguishes between acute lymphoblastic leukemia (cells stain positive) and acute myeloblastic leukemia (cells stain negative).
There are many disadvantages to stain and not staining cells before viewing them. One disadvantage of gram staining is that the cells cannot be alive while being stained, so viewing live stained cells is impossible.
a microscope