0
What else can I help you with?
What happens when you prepare a smear from a agar plate or slant without first placing water on slide?
If you prepare a smear from an agar plate or slant without first placing water on the slide, the cells may not adhere well to the slide, leading to uneven distribution and difficulty in visualization. Adding a drop of water before preparing the smear helps the cells adhere to the slide and spread evenly for better microscopic examination.
How does a smear preparation of cells from a liquid medium differ from preparation of cells from a solid medium?
In liquid medium one starts by placing one or two loopfuls of the liquid medium directly on the slide. From solid media such as nutrient agar. One starts by placing one or two loopfuls of water on the slide and then using an inoculation loop to disperse the organisms in the water. Bacteria growing on solkid media tend to cling to each other and mus be dispensed suficiently by dilutiion in water.
Why is it necessary to excessively rinse the smear with water in experiment identifying bacteria using gram stain?
smear should be rinsed with distill water so that all the small particles attach to the smear will washed away such as some time the crystals of dye are attached to the smear which give the illusion of microbial cell some time, distill water is used because it is free from other microbial cell and ions which can harm the smear.
Why do you air-dry a smear?
Air-drying a smear helps to fix the cells onto the slide, preventing any loss or distortion during further processing steps like staining or examination under a microscope. It also helps to evaporate any excess water, improving the visualization of the cells.
What is the procedural difference between using broth and agar as a source of bacteria for preparing a smear?
If your colonies were grown in broth, you can simply use your loop to collect loopfuls of liquid medium and smear that onto a glass slide. If they were grown on an agar plate you would have to add a few drops of water to the surface of the glass slide.
Why tap water is used for smear washing in staining?
Tap water is used to wash the excess stain from a slide prepared from a smear. You can use tap water instead of distilled water because you aren't worried about a precipitate forming and tap water is much cheaper than distilled.
What is perfect direct fecal smear?
A perfect direct fecal smear is a microscopic examination technique used to identify parasites, bacteria, or other abnormalities in stool samples. It involves placing a small amount of feces on a microscope slide, mixing it with a drop of saline or water, and then covering it with a coverslip. This method allows for the visualization of motile organisms and cellular components, providing quick results for diagnosing gastrointestinal infections. Proper technique and immediate analysis are crucial for accurate results.
Can your water break while getting a pap smear done?
Sure, it's possible for your water to break during a pap smear. There is nothing about the pap smear process that would cause your water to break.
What happens when you prepare a smear from a agar plate or slant without first placing water on slide?
If you prepare a smear from an agar plate or slant without first placing water on the slide, the cells may not adhere well to the slide, leading to uneven distribution and difficulty in visualization. Adding a drop of water before preparing the smear helps the cells adhere to the slide and spread evenly for better microscopic examination.
Should you always use sterilized water for smear preparation?
Yes, when you are performing smear preparation, you should always use sterilized water. This will ensure that nothing contaminates your slide.
Is ascariasis a waterborne disease?
Ascaris is due to fecal-oral contact. So it is either food or water that have been contaminated by fecal material.
What is total coliform fecal coliform and fecal streptococci?
Total coliforms are a group of bacteria found in the environment, including soil and vegetation, as well as in the intestines of warm-blooded animals. Fecal coliforms, a subset of total coliforms, specifically indicate contamination from fecal matter, primarily from mammals. Fecal streptococci are another group of bacteria that also serve as indicators of fecal contamination, particularly from humans and warm-blooded animals. These microorganisms are commonly used in water quality testing to assess the safety of drinking water and recreational waters.
Why does water from bathroom sink taste like fecal matter?
There could be several reasons why water from a bathroom sink tastes like fecal matter, including sewage contamination in the water supply, a buildup of bacteria or algae in the pipes, or a malfunctioning water filtration system. It is important to contact your local water provider to investigate the issue further and ensure the water is safe to drink.
How does typhus attack?
Typhoid fever is transmitted through fecal material. This usually occurs when fecal material is transferred orally through food or water.
What kind of bacteria is in water that can cause pink eye?
Fecal matter
Where is water absorbed from fecal matter before defecation?
Water is primarily absorbed from fecal matter in the large intestine, specifically in the colon. As the contents move through the colon, the walls absorb water and electrolytes, which helps to form solid stool. This process is crucial for maintaining hydration and electrolyte balance in the body. Any remaining water in the fecal matter is expelled during defecation.
How does a smear preparation of cells from a liquid medium differ from preparation of cells from a solid medium?
In liquid medium one starts by placing one or two loopfuls of the liquid medium directly on the slide. From solid media such as nutrient agar. One starts by placing one or two loopfuls of water on the slide and then using an inoculation loop to disperse the organisms in the water. Bacteria growing on solkid media tend to cling to each other and mus be dispensed suficiently by dilutiion in water.
