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You first inoculate your loop with bacteria and streak a few times across your plate, heat your loop on a bunsen
burner to sterilize it and let the loop cool to. Bring your loop back onto where you previously streaked a few times swiping back and forth....heat
loop again to sterilize and let it cool. Then the last and third time take your inoculating loop back on where you streaked last time back and forth a few time across the plate always turning your plate 90 degrees. Always remember to stay close to the bunsen
burner so you are in the sterile area of the flame to prevent any unwanted contamination of your bacteria. After your media is incubated you should notice 3 streak marks and the third streak mark should be the most dilute where the bacterial colonies are separate and easily observed and can be used further, if needed.
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Why use a streak plate to grow a bacterium?
You do a streak plate in order to get isolated colonies. If you inoculate into a slant, you have less surface area to work and less chance of getting isolated colonies. In broth, you'll definitely get growth but you won't know WHAT is growing. You go back into each quadrant (a little) with your loop in order to "dilute" the bacteria and get colonies. Quadrant 1 is pretty think (like a smear on the plate) but by the time you get to Quadrants 3 and 4, you should see more defined colonies and not just a film of bacteria.
What advantage does the streak plate method have over the pour plate method?
A disadvantage of the streak plate technique could be colony isolation problems. If the streaking technique is not done properly or if there is too much of an organism present on the inoculating loop then the cells can cluster and form what looks like one colony but it can actually be a couple colonies (made from a couple cells). This can cause an inaccurate colony forming unit count.
Can you use the word microbiology in a sentence?
yes, as in "I have a degree in microbiology"
Why do you use streak plates?
If you are talking about testing minerals, the streak plate causes powder from the mineral to come of and you rub your finger on the plate after, and see what color the dust the mineral makes is.
When would a microbiologist want to use the streak-to-grow method.?
Plate streaking is often done to isolate a colony of bacteria. For instance, if a broth was grown with 2 or 3 different types of bacteria, it could be streaked out in a "3-phase streak." In a 3-phase streak the initial streak takes up a very small area (we draw a T on the back of the plate, this streak goes in the section above the T). The loop is flamed to kill off any bacteria still on it, then a couple streaks are made out of the original. This grabs some of the bacteria from that concentrated streak and spreads them out. This is repeated once more until the final streaks are less and less concentrated bacteria. When we incubate the plate we'll find lots of growth where the original streak was and less and less as we follow the path of the 3-phases. What you're looking for now is a single colony, off by itself. This can then be scraped off and plated on a separate plate and considered a "pure colony." Another usual time to use streak-to-grow bacteria is when you want to know the quantity or concentration of bacteria. You take maybe 0.1mL of solution and plate it, then count the number of colonies that form. Say you have 56 colonies, now you can say: 56 cfu (colony forming units) -------- 0.1 mL or 560 cfu/mL This is usual when testing to see whether a sterilization technique worked, or if a product is within the regulated levels of bacteria to be released the public, etc etc.
How is a minterals streak determinded?
Streak color is determined by scraping the mineral across a a streak plate, (which is made of unglazed porcelain), and then observing the color of the streak, which is left on the plate. Note that some minerals do not leave a streak, as they are too hard. Thus, it is important to learn other identification methods, to use in conjunction with streak color, in order to identify minerals.
Why use a streak plate to grow a bacterium?
You do a streak plate in order to get isolated colonies. If you inoculate into a slant, you have less surface area to work and less chance of getting isolated colonies. In broth, you'll definitely get growth but you won't know WHAT is growing. You go back into each quadrant (a little) with your loop in order to "dilute" the bacteria and get colonies. Quadrant 1 is pretty think (like a smear on the plate) but by the time you get to Quadrants 3 and 4, you should see more defined colonies and not just a film of bacteria.
How do you know a mineral's streak?
The streak of a mineral can distinguish between two samples that have the same color. The streak is often a different color. To test streak, use a streak plate. This is a piece of unglazed porcelain, like the back side of a tile.
What does a mineral and streak tell you and how do you test for it?
The streak of a mineral can distinguish between two samples that have the same color. The streak is often a different color. To test streak, use a streak plate. This is a piece of unglazed porcelain, like the back side of a tile.
What does a mineral's streak tell and how do you test for it?
The streak of a mineral can distinguish between two samples that have the same color. The streak is often a different color. To test streak, use a streak plate. This is a piece of unglazed porcelain, like the back side of a tile.
What does mineral's streaks tell you and how do you test for it?
The streak of a mineral can distinguish between two samples that have the same color. The streak is often a different color. To test streak, use a streak plate. This is a piece of unglazed porcelain, like the back side of a tile.
How do you use the word microbiology in a sentence?
I can not use microbiology in a sentence.
What is the significance of using a 'dry and hard' agar plate in streak plating?
Why is it impotant to use dry and hard agar for streaking
What advantage does the streak plate method have over the pour plate method?
A disadvantage of the streak plate technique could be colony isolation problems. If the streaking technique is not done properly or if there is too much of an organism present on the inoculating loop then the cells can cluster and form what looks like one colony but it can actually be a couple colonies (made from a couple cells). This can cause an inaccurate colony forming unit count.
Can you use the word microbiology in a sentence?
yes, as in "I have a degree in microbiology"
Why do you use streak plates?
If you are talking about testing minerals, the streak plate causes powder from the mineral to come of and you rub your finger on the plate after, and see what color the dust the mineral makes is.
List two methods for obtaining isolated colonies.?
Use a "streak plate" or a "spread plate". Mediums that allow some organisms to grow and not others can also be used. These types of mediums are called Selective medium.
