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because STR only requires small pieces of DNA (2-5 base pairs long). it is fast and automated wheres RFLP can take up to a month to accomplish. STR is also better because it allows the use of the Polymerase Chain Reaction (PCR). whereas RFLP requires large amounts of non-degraded DNA and automation is not possible.
What else can I help you with?
How is the DNA molecule divided in RFLP?
In RFLP analysis, the DNA molecule is first isolated from the sample. Then, it is digested with restriction enzymes to cut it into fragments at specific sites, creating a pattern of different lengths. These fragments are separated by size using gel electrophoresis, allowing for comparison of the fragment patterns between different samples.
Why do the number of DNA fragments and the length of each fragment produced by RFLP analysis differ from person to person?
As the DNA fragments results from the action of the restriction enzymes and on the other hand mutations alter the sites where the restriction enzymes react therefore there is difference in number and of length of each fragment from person to person.
What is a person's unique RFLP pattern?
sometimes, there isn't enough DNA found at a crime scene to be properly analyzed by forensic scientists. RFLPs are used to make exact copies of the DNA sequence so that it can be better analyzed in the lab.
How do you make a DNA fingerprint?
it can be as little as a single cell if using low copy number (LCN) analysis however if you are looking for results that are a little more reliable its ussially over 1 nano gram, that's using STR (short tandom repeate) analysis. if using something like RFLP (ristriction fragment length polymorphism) it would be alot more then that, however that technique is hardly used anymore. visually repisented the smallest amount of DNA to produce an STR profile is the size of a full stop . i hope this helps thegreatmizuti
What is RFLP used for?
RFLP (often pronounced "rif lip", as if it were a word) is a method used by molecular biologists to follow a particular sequence of DNA as it is passed on to other cells. RFLPs can be used in many different settings to accomplish different objectives. RFLPs can be used in paternity cases or criminal cases to determine the source of a DNA sample. RFLPs can be used determine the disease status of an individual. http://www.bio.davidson.edu/COURSES/genomics/method/RFLP.html
What is the rflps?
Do you mean "RFLP" if so its, restriction fragment length polymorphism. (DNA analysis)
What Is made during an RFLP analysis?
During an RFLP (Restriction Fragment Length Polymorphism) analysis, DNA is digested with restriction enzymes, separated by gel electrophoresis, and transferred to a membrane for hybridization with a probe. The resulting pattern of DNA fragments of varying lengths is visualized to identify variations in DNA sequences between individuals.
Is it possible for two people to have the exact same RFLP banding pattern?
It is highly unlikely for two people to have the exact same RFLP banding pattern due to the vast genetic variability among individuals. RFLP analysis is based on variations in DNA sequences, making it improbable for two unrelated individuals to have identical patterns.
How much DNA is needed to be collected at a scene to produce a DNA fingerprint?
it can be as little as a single cell if using low copy number (LCN) analysis however if you are looking for results that are a little more reliable its ussially over 1 nano gram, that's using STR (short tandom repeate) analysis. if using something like RFLP (ristriction fragment length polymorphism) it would be alot more then that, however that technique is hardly used anymore. visually repisented the smallest amount of DNA to produce an STR profile is the size of a full stop . i hope this helps thegreatmizuti
What can be the main limiting factor in the use of RFLP?
What can be the main limiting factor in the use of RFLP?
In molecular biology RFLP is an acronym for what?
RLFP is an acronym for Resriction Fragment Length Polymorphism. RLFP analysis is used to identify changes in a genetic sequence that occurs at a site where a restriction enzyme cuts. RFLP's can be used to identify specific mutations and also trace inheritance patterns!
What method is used to make DNA fingerprints?
A method known as RFLP (restriction fragment length polymorphism) analysis can be used to make a DNA fingerprint.
What has the author Stephen James Gray written?
Stephen James Gray has written: 'The genotyping of neisseria meningitidis by restriction fragment lengthpolymorphism (RFLP) analysis'
What is better int or str?
It depends on your game and your class.
How do you toggle case of string in c code. Example is TogGle will turn into tOGgLE?
#include<stdio.h> int main() { char str[100]; int i; printf("Please enter a string: "); // gets(str); // fgets is a better option over gets to read multiword string . fgets(str, 100, stdin); // Following can be added for extra precaution for '\n' character // if(str[length(str)-1] == '\n') str[strlen(str)-1]=NULL; for(i=0;str[i]!=NULL;i++) { if(str[i]>='A'&&str[i]<='Z') str[i]+=32; else if(str[i]>='a'&&str[i]<='z') str[i]-=32; } printf("String in toggle case is: %s",str); return 0; }
How is the DNA molecule divided in RFLP?
In RFLP analysis, the DNA molecule is first isolated from the sample. Then, it is digested with restriction enzymes to cut it into fragments at specific sites, creating a pattern of different lengths. These fragments are separated by size using gel electrophoresis, allowing for comparison of the fragment patterns between different samples.
Why do the number of DNA fragments and the length of each fragment produced by RFLP analysis differ from person to person?
As the DNA fragments results from the action of the restriction enzymes and on the other hand mutations alter the sites where the restriction enzymes react therefore there is difference in number and of length of each fragment from person to person.
