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Why is badly degraded genomic DNA a poor source of genes for cloning experiments?
Wiki User
∙ 14y ago
the bases are all degraded, which means that the information needed in the degraded DNa will be unreliable, error-prone, damaged enough to inhibit replication or even if it does replicate, ensure reliable wild type translation of functional protein products
Wiki User
∙ 14y ago
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What are the uses of DNA cloning in Forensic Science?
To produce enough copies of a DNA sample, that identification can be made of the source of the DNA sample.
What is the difference between animal and plant DNA extraction?
Plant DNA extractionPlant genomic DNA is more difficult to extract because of the plant's cell wall, which is removed by homogenization, or by adding cellulase to degrade the cellulose that makes up the cell wall. Also, the metabolites present in the plant cell may interfere with genomic DNA extraction by contaminating the DNA sample during the precipitation process.Animal DNA ExtractionPeripheral blood leukocytes are a main source of animal genomic DNA, but sample collection is difficult as blood must be withdrawn from the animal. Blood contains a range of compounds like proteins, lipids, white blood cells, red blood cells, platelets, and plasma, which can contaminate the DNA sample. The primary contaminant of animal DNA extracted from blood samples is heme, the non-protein component of hemoglobin.DifferencesThe differences between plant and animal DNA lie in the sequence of bases in the helix. Compounds found in plant cells are absent in animal cells, and DNA base sequences reflect this, as the genomic plant DNA is often larger than animal DNA. These differences affect extraction methods, as it impacts on yield and purity of DNA.
What is a fingerprinting source?
Fingerprinting is a scientific technique based on the fact that each person has a unique, identifying fingerprint. Fingerprint analysis has a variety of uses including criminal investigation, security access and genomic research. For example a fingerprint source could be taken from a crime scene where a thief has used a door. The door handle will hold a record of the theifs fingerprint and when analysed can be compared against a database to see if the criminal has comitted any previous crimes. This makes finding and arresting the suspect much easier. Hope this helps
Why do molecular biologists use yeast as opposed to bacteria for expressing genes of interest?
molecular biologists can avoid eukaryotic-prokaryotic incompatibility by using eukaryotic cells such as yeasts, rather than bacteria, as hosts for cloning and/or expressing eukaryotic genes of interest. source: Campbell Biology 7th edition
The energy in food molecules such as glucose is converted into what?
Its been degraded to two molecules of pyruvate.
What is the role of 2-mercaptoethanol in the isolation of genomic DNA from a plant source?
It is an antioxidant.
What is an example of an beneficial mutation?
Cloning animals such as cows so we have an almost infinite food source.
What is cloning in Photoshop?
To clone means to replace pixels on area where you paint with pixels from sampled (source) area (first you must choose source area).
Who discovered conducted experiments in combining elements?
John Dalton conducted experiments combining elements with atoms.
Why is cloning a form of reproduction?
Sexual reproduction requires the mixture of DNAs from two different sources(i.e. you got a set of chromosomes from you mother and one from your father). Asexual reproduction involves a copying of just one source of DNA. In cloning, you take DNA from just one source and so it is asexual.
Why is cloning a form of asexual reproduction?
Sexual reproduction requires the mixture of DNAs from two different sources(i.e. you got a set of chromosomes from you mother and one from your father). Asexual reproduction involves a copying of just one source of DNA. In cloning, you take DNA from just one source and so it is asexual.
What are the uses of DNA cloning in Forensic Science?
To produce enough copies of a DNA sample, that identification can be made of the source of the DNA sample.
What is cloning an image using Photoshop?
Cloning is replicating pixels from chosen source to area where are you painting with Clone Stamp Tool. There are and other tools to clone pixels from one image area to another: Healing Brush Tool and Patch Tool.
Why are people against cloning?
Some people are worried that cloning technology might eventually be used for immoral purposes. People might, in theory, be mass-produced as slaves or soldiers. People could be cloned as a source of spare organs for the wealthy.
Why is it important to use water from the same source and at the same temperature for both experiments?
so it can be a fair test
What do you use Nuclear Fusion for?
At present it is only used for experiments, to try to develop a reliable power source
What energy source does the ISS run on?
The primary power source is solar power from the large arrays connected to the station. There are also two backup generators and smaller fuel cells for various experiments.
