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If this is the question asked in your lab manual, welcome to the club. That's because diluted cultures produce little blue men and some pink powders also lots of chocolates when they are put on a spread plate and it yields a successful happy life for you. Tell that to your teacher and you'll get an AA.

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12y ago
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9y ago

It is necessary because more than 200 cells are too many to count accurately. Less than 100 doesn't give you enough of a sample.

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6y ago

It will be impossible to count the numbers if they are not first diluted. It also gives a number that you can compare to others.

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Q: Why is it necessary to use only diluted cultures that contain 100 to 200 cells for successful spread plate?
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Related questions

Why it necessary to use only diluted cultures that contain 100 to 200 cells for a successful plate counting?

It is necessary to use only diluted cultures that contain 100 to 200 cells for a successful plate counting because above that the colonies will be too dense on the plate and counting them will be difficult. Also, many of the colonies will merge.


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To clean a water cooler dispenser, remove all filters and run 35% food grade hydrogen peroxide diluted with 11 times as much water through the dispenser. It is necessary to use food grade hydrogen peroxide as it does not contain the harmful stabilizers contained in regular 3% hydrogen peroxide. The dilution step is necessary because otherwise the peroxide will be too caustic.


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