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Probably because that is where the gram negative bacteria have found a food source. Lactose is not the perfered diet, but it works and they are most likely there because they can tolerate the environment, and survive (on lactose) when their #1 food source is not available.

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Quantity of lactose in levine eosin methylene blue agar?

Levine eosin methylene blue agar does not contain lactose. It is a selective and differential medium used for the isolation and differentiation of gram-negative enteric bacteria based on their ability to ferment lactose. The agar contains lactose as a substrate for fermentation, but the actual quantity of lactose present in the agar may vary depending on the manufacturer and the specific formulation of the medium.


Can Serratia grow on Hektoen Enteric Agar?

Yes. Serratia is a Gram negative rod from the Enterobacteriaceae family. The Hektoen Enteric agar allows the growth of Gram negative bacteria while inhibiting growth of Gram positive bacteria.


Does providencia bacteria ferment lactose?

Yes, Providencia bacteria have been shown to ferment lactose in laboratory settings. However, not all strains may possess this capability, so it's important to confirm lactose fermentation through biochemical testing.


Differentiate a coliform an enteric that in not a coliform and enterococci Give an example of an organism in each group?

Coliforms are a type of bacteria commonly found in the intestines of warm-blooded animals, such as E. coli. Enterococci are also intestinal bacteria, but they do not produce gas from lactose fermentation like coliforms. An example of a non-coliform enteric bacteria is Enterobacter aerogenes. An example of an enterococci is Enterococcus faecalis.


Why isn't a pH indicator needed in the lactose broth fermentation tubes?

A pH indicator is not needed in lactose broth fermentation tubes because the fermentation of lactose by bacteria produces acid as a byproduct, lowering the pH of the broth. This drop in pH can be visually observed as a color change in the medium, indicating lactose fermentation.


Does Morganella Morganii ferment lactose?

no, it shows negative for lactose fermentation on MacConkey's agar


What is the role of lactose in EMB?

In Eosin Methylene Blue (EMB) agar, lactose serves as a fermentable carbohydrate that allows for the differentiation of lactose-fermenting bacteria from non-fermenters. Lactose fermenters, such as Escherichia coli, produce acid during fermentation, resulting in a color change in the medium due to the pH indicators eosin and methylene blue. This leads to the formation of dark purple colonies, while non-fermenters typically produce colorless or light-colored colonies. Thus, lactose in EMB is crucial for identifying and isolating lactose-fermenting enteric bacteria.


What is the function of an operator in bacteria?

The function of an operator in bacteria can be defined as the required for the transport and metabolism of the lactose in Escherichia coli and some other enteric bacteria. It consist of three adjacent structural genes.


What bacteria grows in a medium of lactose fermentation?

It is always an unnamed substance that u can call anything


What happens when the pH indicator is left out of macConkey agar?

If the pH indicator is left out of MacConkey agar, the medium will lose its ability to indicate lactose fermentation visually. Normally, the pH indicator (neutral red) changes color in response to acid production from lactose fermentation, allowing for the differentiation of lactose-positive bacteria, which turn pink, from lactose-negative bacteria, which remain colorless. Without the indicator, it becomes difficult to distinguish between these bacterial groups, compromising the effectiveness of the medium for selective isolation and identification.


Give result lactose test for bacteria citrobacter diversus?

Citrobacter diversus is capable of fermenting lactose, therefore it would test positive for lactose fermentation in biochemical tests. This bacterium produces acid and gas during lactose fermentation, which can be detected using appropriate methods such as the MacConkey agar test.


Why isn't a pH indicator needed in lactose broth fermentation tubes for detecting coliform bacteria?

A pH indicator is not needed in lactose broth fermentation tubes for detecting coliform bacteria because the presence of coliforms is indicated by gas production from lactose fermentation rather than pH changes. Coliform bacteria, such as E. coli, ferment lactose, producing acid and gas; however, the gas bubbles trapped in the inverted Durham tube provide a clear, direct visual cue of fermentation. The focus is on the gas indicator rather than pH changes, simplifying the detection process.