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Why isn't a pH indicator needed in the lactose broth fermentation tubes?
Wiki User
∙ 10y ago
not sure bro
Wiki User
∙ 10y ago
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Explain the results in the sterilized and not-sterilized broth?
Sterilized broth is clear, meaning no bacterial growth. Non-sterilized broth is cloudy.
Why didn't we inoculate MUG agar directly and bypass lactose broth?
The intended use of Nutrient Agar with MUG is for detecting an enumerating E. Coli in water. This lab wasn't looking specifically and only for e.coli. It was looking for other types of coliforms as well. this is my best guess
Why is nutrient broth better than peptone broth?
Nutrient broth contains beef extract which acts as sources of amino acids, peptides, nucleotides, organic acids, vitamins and mineralsthat peptone broth don't contain? :)
How long has broth been in our culture?
Broth has been in our culture since the beginning of time.
What is ratio between blood and broth in blood broth?
blood should be 5% by solution
Why isn't a pH indicator needed in the lactose broth fermentation tube?
not sure bro
When using the phenol red carbohydrate broth fermentation test at what pH is the pH indicator yellow?
below 6.8
What reaction would you predict from an organism growing in litmus milk that has the following results in other media?
Assuming that you are asking about other media such as milk agar, PR lactose broth, purple lactose broth, and PR glucose broth, the answer you are looking for is as follows: A. A clear zone around the growth on a milk agar plate: indicates hydrolysis of casein, so should be clarification of medium present B. A/- in PR lactose broth: indicates lactose fermentation with acid and no gas produced, so should be pink litmus with acid clots and reduction of litmus in lower portion of medium C. A/G in purple lactose broth: indicates lactose fermentation with acid and gas produced, so should have pink litmus on top, acid clot, reduction of litmus, and fissures in the clot D. K in PR glucose broth: indicates peptone degradation with alkaline end products, so litmus should be blue
What are the essential ingredients of a carbohydrate broth?
nutrient broth + 0.5% particular carbohydrate + indicator
Why do you need to have an impeller in fermentor?
To mixed the culture broth during fermentation
Composition of double strength lactose broth?
ne abba answer chepamante,,nannu adgutunavera
Saline lactose broth sterilization method?
If the broth contains only saline and lactose, it can be autoclaved. However, it it contains peptide growth factors depending on the application, it will have to be filtered prior to use since protein-containing media is sensitive to heat.
Is there a sentence with broth in it?
Chicken broth is needed in the Thanksgiving gravy.
Why is it necessary to record the results of carbohydrate fermentation tests no later than 48 hours after inoculation?
During prolonged incubation periods, the carbohydrate supply is exhausted and many bacteria will begin growing oxidatively on the peptones in the broth, altering the color of the pH indicator.
Difference between solid state fermentation and submerged fermentation?
Submerged fermentation:" The organism which can grow under the beneath the surface of the medium is called " Ex.. liquid medium (Nutrient broth) solid state-fermentation : "Organisms which can grow on the surface of the medium is called solid state fermentation" Ex.. solid medium (Nutrient agar)
What has the author Timothy Walford written?
Timothy Walford has written: 'The extraction of microbial metabolites from fermentation broth[s] using compressed carbon dioxide'
What is shake flask fermentation?
Shake Flask fermentation is a cheapest ans simplest technique to grow bacteria or fungi, aerobically, in small volumes of nutrient broth. The broth is poured into erlenmeyer flasks equipped with cotton-wool stoppers, and autoclaved. After cooling, some microbes are "seeded" into the flask, and it is placed on a Shaker machine. The shaking agitates the content and so ensures aeration, so that the microbes could breathe.
