Double strength lactose broth is used to selectively culture lactose fermenting bacteria. It contains a higher concentration of lactose compared to regular lactose broth, allowing for more efficient differentiation of lactose fermenters from non-fermenters based on acid production. This medium is often used in microbiology laboratories for various tests and experiments.
In presumptive tests for coliforms, lactose broth is preferred over glucose broth because many coliforms can ferment lactose, producing gas and acid, which are indicators of their presence. While glucose can also be fermented by some bacteria, it is less specific for coliforms. The use of lactose broth helps ensure that the test is more selective for lactose-fermenting organisms, making it a better choice for detecting coliforms in water and food samples.
Assuming that you are asking about other media such as milk agar, PR lactose broth, purple lactose broth, and PR glucose broth, the answer you are looking for is as follows: A. A clear zone around the growth on a milk agar plate: indicates hydrolysis of casein, so should be clarification of medium present B. A/- in PR lactose broth: indicates lactose fermentation with acid and no gas produced, so should be pink litmus with acid clots and reduction of litmus in lower portion of medium C. A/G in purple lactose broth: indicates lactose fermentation with acid and gas produced, so should have pink litmus on top, acid clot, reduction of litmus, and fissures in the clot D. K in PR glucose broth: indicates peptone degradation with alkaline end products, so litmus should be blue
If the broth contains only saline and lactose, it can be autoclaved. However, it it contains peptide growth factors depending on the application, it will have to be filtered prior to use since protein-containing media is sensitive to heat.
The nutrient broth is typically used to grow and culture bacteria, not for consumption. Since it is not intended to be ingested and is only used as a growth medium, it does not need to be cooked. Heating the broth could destroy the nutrients or alter its composition, which would impact its effectiveness in supporting bacterial growth.
Double strength lactose broth is used to selectively culture lactose fermenting bacteria. It contains a higher concentration of lactose compared to regular lactose broth, allowing for more efficient differentiation of lactose fermenters from non-fermenters based on acid production. This medium is often used in microbiology laboratories for various tests and experiments.
In presumptive tests for coliforms, lactose broth is preferred over glucose broth because many coliforms can ferment lactose, producing gas and acid, which are indicators of their presence. While glucose can also be fermented by some bacteria, it is less specific for coliforms. The use of lactose broth helps ensure that the test is more selective for lactose-fermenting organisms, making it a better choice for detecting coliforms in water and food samples.
A pH indicator is not needed in lactose broth fermentation tubes because the fermentation of lactose by bacteria produces acid as a byproduct, lowering the pH of the broth. This drop in pH can be visually observed as a color change in the medium, indicating lactose fermentation.
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Assuming that you are asking about other media such as milk agar, PR lactose broth, purple lactose broth, and PR glucose broth, the answer you are looking for is as follows: A. A clear zone around the growth on a milk agar plate: indicates hydrolysis of casein, so should be clarification of medium present B. A/- in PR lactose broth: indicates lactose fermentation with acid and no gas produced, so should be pink litmus with acid clots and reduction of litmus in lower portion of medium C. A/G in purple lactose broth: indicates lactose fermentation with acid and gas produced, so should have pink litmus on top, acid clot, reduction of litmus, and fissures in the clot D. K in PR glucose broth: indicates peptone degradation with alkaline end products, so litmus should be blue
If the broth contains only saline and lactose, it can be autoclaved. However, it it contains peptide growth factors depending on the application, it will have to be filtered prior to use since protein-containing media is sensitive to heat.
Selenite-F Broth Approximate Formula* Per Liter Purified Water Pancreatic Digest of Casein ....................................................5.0 g Lactose ......................................................................................4.0 g Sodium Selenite ........................................................................4.0 g Sodium Phosphate ..................................................................10.0 g *Adjusted and/or supplemented as required to meet performance criteria.
To make chicken broth using bouillon powder, dissolve the bouillon powder in hot water according to the package instructions. You can adjust the strength of the broth by adding more or less powder.
strength comes from the inside. You have to find it by yourself.
The nutrient broth is typically used to grow and culture bacteria, not for consumption. Since it is not intended to be ingested and is only used as a growth medium, it does not need to be cooked. Heating the broth could destroy the nutrients or alter its composition, which would impact its effectiveness in supporting bacterial growth.
The penicillium is grown in a broth of corn steep liquor, lactose, and calciumcarbonate. Filter the broth through a qualitative paper filter to remove the pencillium. Add activated carbon to absorb the pencillin (allow carbon to soak for 15min). remove carbon via filter and place in acetate.
Nutrient agarPeptone - 5 g/LMeat extract - 1 g/LYeast extract - 2 g/LSodium chloride - 5 g/LAgar - 15 g/LpH - 7.0 ± 0.2Storage temperature - 2-8°CNutrient broth is a liquid formulation that does not contain agarNutrient Borth are used for enrihment of specified bateria like E,coli and salmnella