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For classification and identification purposes. Gram+ stain a dark purple/blue color while Gram - stain a red/pink color. This is due to the fact that Gram-positive cells have more peptidoglycan in their cell wall. Gram-negative cells don't have a think layer of peptidoglycan. They do have an outer layer of lipopolysaccharides and proteins which the Gram + does not have.
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What would be the result of overheating the bacterial smear?
Overheating the bacterial smear can result in distortion or destruction of the bacterial cells, making it difficult to observe them under the microscope. This can lead to inaccurate or inconclusive results when trying to identify the bacteria present on the smear.
What is the Result of over heating the bacterial smear?
When you heat the bacteria more than three times on the flame of Bunsen burner, the bacteria will damage and if you stain this damaged bacteria, the shape of bacteria is not typical and sometimes you just see the residue of stain on the slide.
How would you define a properly prepared bacterial smear?
the bacteria are evenly spread out on the prepared slide in such a concentration that they are adequately separated from one another bacteria are not washed off the slide during staining bacterial form is not distorted
What happens when you prepare a smear from a agar plate or slant without first placing water on slide?
If you prepare a smear from an agar plate or slant without first placing water on the slide, the cells may not adhere well to the slide, leading to uneven distribution and difficulty in visualization. Adding a drop of water before preparing the smear helps the cells adhere to the slide and spread evenly for better microscopic examination.
What is the purpose of covering the smear with bibulous paper when performing the endospore stain?
Covering the smear with bibulous paper during the endospore stain process helps to wick away excess stain and prevent the slide from drying out. This ensures that the endospores are properly stained and the background is clear for observation under the microscope.
What would be the result of overheating the bacterial smear?
Overheating the bacterial smear can result in distortion or destruction of the bacterial cells, making it difficult to observe them under the microscope. This can lead to inaccurate or inconclusive results when trying to identify the bacteria present on the smear.
What are the characteristics of good bacterial smear?
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How do you prepare a heat fixed smear?
To prepare a heat-fixed smear, start by placing a small drop of the specimen (such as bacterial culture) on a clean glass slide. Using a sterile loop or stick, spread the drop evenly to create a thin film. Allow the smear to air dry completely, then pass the slide through a flame briefly to fix the cells to the slide, ensuring not to overheat and damage the sample. Once cooled, the slide is ready for staining and microscopic examination.
Is a bacterial infection test the same as a chlamydia test?
Testing for chlamydia is very specific. A regular bacterial culture or wet smear will not detect chlamydia.
Why does the physician take a Pap smear?
Such swab tests are used to check for gonorrhea and chlamydia, or bacterial vaginitis, which is a bacterial infection resulting in inflammation of the vagina.
What is released by bacterial infection?
What method is used to prepare potassiumsulfate
Give the reason for passing bacterial smear through the flame before staining?
Passing the bacterial smear through the flame before staining is done to heat-fix the bacteria onto the slide, making them adhere firmly and preventing them from washing off during the staining process. Heat fixing also kills the bacteria, which helps in the preservation of their cellular structures for visualization under the microscope.
Which of these algae are used to prepare a medium for bacterial culture?
Agar, a type of red algae, is commonly used to prepare solid culture media for bacterial growth. It solidifies at room temperature, providing a stable surface for bacterial colonies to develop.
What is the Result of over heating the bacterial smear?
When you heat the bacteria more than three times on the flame of Bunsen burner, the bacteria will damage and if you stain this damaged bacteria, the shape of bacteria is not typical and sometimes you just see the residue of stain on the slide.
How would you define a properly prepared bacterial smear?
the bacteria are evenly spread out on the prepared slide in such a concentration that they are adequately separated from one another bacteria are not washed off the slide during staining bacterial form is not distorted
What is cocobacilli in a pap smear?
Coccobacilli on a pap smear result is informational. If the woman is comlplaining fo vaginal discharge and odor, treatment for bacterial vaginosis is offered. If she has no complaints, treatment is not needed.
At what total magnification should you begin to look at a sample?
Depending on what microscopy you are doing.. Bacterial microscopy starts with 40x and Blood smear microscopy at 10x.
