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For classification and identification purposes. Gram+ stain a dark purple/blue color while Gram - stain a red/pink color. This is due to the fact that Gram-positive cells have more peptidoglycan in their cell wall. Gram-negative cells don't have a think layer of peptidoglycan. They do have an outer layer of lipopolysaccharides and proteins which the Gram + does not have.
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∙ 14y ago
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∙ 12y agoIt spreads the bacteria so that it's not all in one large clump. This makes the bacteria easier to view under a microscope, or helps it to grow if that's what you are doing.
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∙ 11y agois a simple stain.
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∙ 14y agothere is an advantage?
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∙ 12y agoto giv it contrast
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What would be the result of overheating the bacterial smear?
your sample can destroy the bacteria and it will appear shapeless or enlarged when you overheat the bacterial smear
What is the purpose of covering the smear with bibulous paper when performing the endospore stain?
This paper will aid in keeping the stain over the bacterial smear.
How would you define a properly prepared bacterial smear?
the bacteria are evenly spread out on the prepared slide in such a concentration that they are adequately separated from one another bacteria are not washed off the slide during staining bacterial form is not distorted
What is the purpose of simple staining procedure?
In simple staining the bacterial smear stained with single dye or reagent which bring the distinctive contrast between organism and its background. PROCEDURE: 1 Prepare bacterial smear by placing loopfull culture of bacteria on slide. 2 Allow the slide to dry and heat fix it over flame. Do not heat extreamly, it can demage the shape or structure of bacteria. 3 Now flood the smear with methylene blue for 1-2 minutes. 4 Gently wash the smear with distill water to remove excess stain and dry it slowly with tissue paper. 5 Examine the slide in 100x or oil-immersion for the morphology of bacteria.
What is the purpose of staining the smear in malachite green during spore staining?
the purpose of boiling of smear in malachite green is to forces a stain to penetrate the endospore wall, it is necessary to heat the slide and the stain to prod the wall to allow the stain to enter.
What would be the result of overheating the bacterial smear?
your sample can destroy the bacteria and it will appear shapeless or enlarged when you overheat the bacterial smear
What are the characteristics of good bacterial smear?
no thing
What is the purpose of covering the smear with bibulous paper when performing the endospore stain?
This paper will aid in keeping the stain over the bacterial smear.
Give the reason for passing bacterial smear through the flame before staining?
It dries the smear and fixes the cells to the slide
Why does the physician take a Pap smear?
Such swab tests are used to check for gonorrhea and chlamydia, or bacterial vaginitis, which is a bacterial infection resulting in inflammation of the vagina.
Is a bacterial infection test the same as a chlamydia test?
Testing for chlamydia is very specific. A regular bacterial culture or wet smear will not detect chlamydia.
What is a common mistake made when preparing a bacterial smear that results in large aggregates of bacteria piled on top of one another?
This is often the result of using far too many bacteria. You only need to take a very small loop from the bacterial culture to make a clear smear.
What is released by bacterial infection?
What method is used to prepare potassiumsulfate
Which of these algae are used to prepare a medium for bacterial culture?
Red Algae ?
What happens if you don't heat fix a smear prior to staining of a bacterial slide?
smear will be washed( no smear will be left on the slide)
How do you examine live bacteria microscopically?
You observe bacterial smear using oil immersion lens with a magnification of 100x.
During the performance of the simple staining procedure you failed to heat fix your EColi smear preparation Upon microscopic examination how would you expect this slide to differ from correct slide?
The bacterial smear will wash away during the staining procedure. This is avoided by heat fixation, during which the bacterial proteins are coagulated and fixed to the glass surface.
