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When you heat the bacteria more than three times on the flame of Bunsen burner, the bacteria will damage and if you stain this damaged bacteria, the shape of bacteria is not typical and sometimes you just see the residue of stain on the slide.
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What is the purpose of covering the smear with bibulous paper when performing the endospore stain?
This paper will aid in keeping the stain over the bacterial smear.
What is the purpose of simple staining procedure?
In simple staining the bacterial smear stained with single dye or reagent which bring the distinctive contrast between organism and its background. PROCEDURE: 1 Prepare bacterial smear by placing loopfull culture of bacteria on slide. 2 Allow the slide to dry and heat fix it over flame. Do not heat extreamly, it can demage the shape or structure of bacteria. 3 Now flood the smear with methylene blue for 1-2 minutes. 4 Gently wash the smear with distill water to remove excess stain and dry it slowly with tissue paper. 5 Examine the slide in 100x or oil-immersion for the morphology of bacteria.
Why do you air-dry a smear?
To avoid denaturing and destroying the smear.
Why can't a smear be gently heated over a a flame to speed up the drying process?
it can kill the cells
What happens when you leave a stain on the bacterial smear for too long?
If the bacterial cell are under stained then they will loose the stain of dye when wash with alocohol or may be simply by water which then cause a problem in identifying the cell type as e.g. in case of gram staining if the cell do not stain properly with methylene blue then they will loose the stain when washed and will counter stain with saffranin so the gram +ve will show the gram -ve colour.
What is the purpose of covering the smear with bibulous paper when performing the endospore stain?
This paper will aid in keeping the stain over the bacterial smear.
A computer with its cover removed can result in?
Over heating Actually - if you remove the cover from a computer - you are at risk of electric shock - NOT the computer over heating as the previous answer said !
Low car coolant?
Can result in freezing in the winter and over-heating in the summer.
What is a sentence for smear?
The smear test will determine if you have cervix cancer.This is nothing more than a childish smear campaign by a jealous individual.
Can a temperature sending unit cause over heating?
It won't cause over heating.....but it can go bad and send a signal that the engine is over heating when it is not.
What is the consequence of leaving a stain on the bacterial smear too long over staining?
Leaving a stain on too long generally stains everything; leaving little contrast. Leaving a stain on not long enough doesn't stain enough; leaving little contrast.
What would we observe when we place a metal lid over a glass of hot drink?
Placing a metal lid over a glass of hot drink would result in heating up of the metal.
How do I make a silly sentence with the word smear?
The clown used his coat sleeve to smear the cream pie all over the other clown's face.
What is meant by heat fixing a bacterial smear?
to allow the excess water to dry out before heating. if heated right after, the water would cause the smear to overheat and denature some features in the stain. and those features would no longer be seen
What is the purpose of simple staining procedure?
In simple staining the bacterial smear stained with single dye or reagent which bring the distinctive contrast between organism and its background. PROCEDURE: 1 Prepare bacterial smear by placing loopfull culture of bacteria on slide. 2 Allow the slide to dry and heat fix it over flame. Do not heat extreamly, it can demage the shape or structure of bacteria. 3 Now flood the smear with methylene blue for 1-2 minutes. 4 Gently wash the smear with distill water to remove excess stain and dry it slowly with tissue paper. 5 Examine the slide in 100x or oil-immersion for the morphology of bacteria.
What are some of the causes that would result in a cylinder head to crack on a ford mustang?
Metal fatigue? Improper tightening procedure Improper torque Over heating
How do you make a mask representing Will Smith?
smear chocolate all over your face
