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Why should the swab first be moistened in sterile PBS before sampling an area and transferring to an agar plate?
Wiki User
∙ 10y ago
it is need to miosten the cotton swab with saline solution to facilitate the capture of whatever microorganisms are present. :))))
Wiki User
∙ 10y ago
Wiki User
∙ 10y agoThere are different reasons, and it depends on what you are sampling. For example, if you want to take a rectal swab, you would wet the swab for patient comfort and to lessen the chance that the absorbent tip of the swab would fall off/get stuck when testing. If you are taking a swab of a dry surface, wetting the swab would allow more of the surface contaminants to be picked up by the swab (think of wetting a towel and running it accross a dirty table vs. using a dry towel)
Wiki User
∙ 13y agoyou must be taking medical microbiology. I have the exact same question in my book. lol. Somebody answer this for us please.
Our micro prof told us that it is just to facilitate the capture of whatever microorganisms are present.
Wiki User
∙ 12y agoIt is first moistened for easy collection of the samples
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What is a sentence using the word sterile?
the sergen kept his equpment sterile before the sergery
Lists of aseptic technique you used in lab?
There are different types of Aseptic Techniques such as uphold of sterilastion. This is when you insure that the sterile sections in labs are kept sterile, by using sterile equiptment (such as gloves, etc) and washing hands for at least 2-5mins before entering sterile environments. Another technique is when dealing with sterile equiptment, not only do you use gloves, but ensure that you use the procedure of handeling equiptment in a certain way. Tilt the testube to the side for example, when you are screwing and unscrewing the lid of a container, using the side of your hands, not touching the rim with yor fingers etc. This should help a bit :)
What are perflurochemicals?
Perflurochemicals (perflurocarbons) are being researched into as an artificial blood Synthetic, and completely inert Sterile Cheap to produce - compared to real blood Can dissolve 5 times more oxygen then blood Free of biological material - sterile Needs to be combined with other agents before being used in the bloodstream (eg. Lecithin)
Why is it necessary to touch a sterile part of the agar with the loop before touching the bacterial growth?
It is a way to check that the loop isn't hot since you flamed it just before. I have seen some actually sizzle.
Describe the methods that you can use to obtain a pure culture and state the significance?
A pure culture is usually derived from a mixed culture (one containing many species) by transferring a small sample into new, sterile growth medium in such a manner as to disperse the individual cells across the medium surface or by thinning the sample manyfold before inoculating the new medium. Both methods separate the individual cells so that, when they multiply, each will form a discrete colony, which may then be used to inoculate more medium, with the assurance that only one type of organism will be present
What is the purpose of shaking the sample before transferring the sample into a sterile McCartney bottle?
shaking the sample before transferring it into a sterile universal bottle is an important step in many microbiological experiments as it helps to ensure that the sample is well-mixed and that any microorganisms present are evenly distributed throughout the sample.
Which subgrade uses moistened burlap before placing concrete on it?
day
Why loop and cap should not be laid on workbench while transferring inoculum?
To prevent contamination. Once you have flamed your loop and cap, do not lay it down, blow on it, touch it with your fingers, or touch it to any surface other than your inoculum or the sterile media.If you do, you must reflame the loop before proceed to inoculation to re-sterile it again.
What is a sentence using the word sterile?
the sergen kept his equpment sterile before the sergery
What is the difference between sterile and non-sterile dressings?
sterile gloves are clean never before used gloves, while non-sterile gloves have been used but are clean
What is the difference between sterile and non-sterile gauze?
One is guaranteed to have no microorganisms on it inside the sterile packaging, the other is clean but is not guaranteed to have no microorganisms on it.
Advantages and dis advantages of sampling?
There are many advantages and disadvantages of sampling. These advantages include being able to try what you need before you buy.
Why had Garvey asked Cole to taste each ingredient before sampling the cake?
will this is what i think garvey asked cole to taste each ingredient before sampling the cake because they didn't know that how they tasted.
Splitting a population into groups with similar characteristics before sampling is called?
stratificatin
What is direct sampling?
When you taste the wine first secretly before buying at the market.
What does the central limit theorem say about the shape of the sampling distribution of?
The central limit theorem can be used to determine the shape of a sampling distribution in which of the following scenarios?
The Pokemon from koeon is it easy to lv up?
No easier than it was before transferring it.
