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Why should the test tube caps not be placed on the counter during a procedure?
Wiki User
∙ 14y ago
Even though you disinfect a lab bench before working on it, you cannot guarantee it is free of microrganisms (think air currents, etc.). If you place a cap on your work bench there is a good chance that it will pick something up and wind up contaminating whatever is in your test tube when you re-cap it. Conversely, lets say you have a broth culture in your test tube and it sloshes around and gets into the cap. When you take the cap off and place it on the bench you will be spreading culture onto your work area. From there you can wind up with culture on you (make sure you are wearing a lab coat!), cross-contaminate samples if you get it on your gloves, etc. In short good aseptic technique requires you work close to your flame and hold the cap in whichever hand you are not holding the test tube (it is much easier to re-cap this way).
Wiki User
∙ 14y ago
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