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Streaking is to produce single colonies. If we are digging to the agar while streaking the microbes, instead of growing on the agar surface grows in the subsurface as well. These colonies may be difficult to isolate.
Wiki User
∙ 13y ago
Wiki User
∙ 9y agoIt is best to use an inoculating loop when making smears from liquid media because they allow you to pick up enough media to make a good smear. Needles do not pick up enough media.
Wiki User
∙ 14y agoDon't allow the loop to penetate into the agar as that will yield a clump of bacteria
Wiki User
∙ 15y agoBecause the colonies will run together.
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Function wire loop?
The function of a wire loop is to transfer small amount of bacterial culture into broth. It is also used to move an agar plate for its continued growth.
Why is it necessary to touch a sterile part of the agar with the loop before touching the bacterial growth?
It is a way to check that the loop isn't hot since you flamed it just before. I have seen some actually sizzle.
When bacteria from a throat swab are streaked on blood agar why is the agar stabbed several times with the loop?
first of all the method of stabbing the agar is no only for throat sample but can be for all specimen except for liquid samples such as urine. the second thing is by stabbing the loop, you can save your time to sterile the loop back. and the other thing is to make sure the loop are no hot anymore..
How is air contamination prevented when an inoculating loop is used to introduce or take a bacterial sample from an agar plate?
The plate cover is raised and held diagonally to protect the plate surface from any contamination in the air.
Why did you use an inoculating loop instead of a needle to make the transfers from the culture plates to the culture tubes?
A inoculating loop is used for transfers from culture plates to culture tubes instead of the inoculating needle because the needle could puncture the agar in tube. The loop is much easier as well to get liquid amount into the tube.
How do you determine when a loop is cool?
Touch it to the sterile agar in a plate. If it melts the agar, it is still hot. If it doesn't melt the agar, then it is cool enough.
Function wire loop?
The function of a wire loop is to transfer small amount of bacterial culture into broth. It is also used to move an agar plate for its continued growth.
Why is it necessary to touch a sterile part of the agar with the loop before touching the bacterial growth?
It is a way to check that the loop isn't hot since you flamed it just before. I have seen some actually sizzle.
Why that you should cool first the inculating loop or needle before using?
"because otherwise you will kill the bacteria from the heat, and you may also melt the agar jelly that you are innoculating the bacterium onto."
When bacteria from a throat swab are streaked on blood agar why is the agar stabbed several times with the loop?
first of all the method of stabbing the agar is no only for throat sample but can be for all specimen except for liquid samples such as urine. the second thing is by stabbing the loop, you can save your time to sterile the loop back. and the other thing is to make sure the loop are no hot anymore..
Why are cells allowed to swim into and out of the loop's water film rather than scraping the agar surface of growth and then emulsifying cells on the slide?
The ability of cells being allowed to swim into and out of the loop's water film rather than scraping the agar surface of growth and then emulsifying cells on the slide is due to its morphology. The path chosen is due to the cell's make up.
What are the steps in properly streaking a plate?
When we have to isolate a specific microbial species from their mix culture or to grow any microbe on solid surface for their further studies then they can be grow on a culture medium containing a gel like substance known as agar which produce disticnt microbial colonies when inoculate in a petri dish containing the growth medium. The way by which the inoculation of microbial sample done is called a streak plate method in which the microbial sample is streak with the help of inoculating loop on the agar plate firmly, in the way so that the cell can be isolated. There are two more method to incoulate the microbial sample that are: pour plate and spread plate techniques.
How is air contamination prevented when an inoculating loop is used to introduce or take a bacterial sample from an agar plate?
The plate cover is raised and held diagonally to protect the plate surface from any contamination in the air.
When getting inoculums from a slant why is it necessary to touch a sterile part of the agar with the loop before touching the bacterial growth?
It is a way to check that the loop isn't hot since you flamed it just before. I have seen some actually sizzle.
Why did you use an inoculating loop instead of a needle to make the transfers from the culture plates to the culture tubes?
A inoculating loop is used for transfers from culture plates to culture tubes instead of the inoculating needle because the needle could puncture the agar in tube. The loop is much easier as well to get liquid amount into the tube.
Agar agar grain found in Germany?
Agar, or agar-agar, is not a grain, but rather an extract of seaweed. Agar translates to German as Agar-Agar Try whole- or health-food stores
What factors would account for an absence of growth on a streak plate?
* Wrong temperature in the incubator * Not enough time in the incubator * No culture was obtained on the inoculating loop * Wrong type of agar for optimum growth (nutrients,etc.)
