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In a paper on the catalytic activity of an enzyme, in which section would you find detailed information on how the enzyme was prepared and handled in the experiment?

materials and methods


The restriction enzyme used in constructing hybrid molecules of certain gene sequences and plasmid DNA acts by?

recognizing specific DNA sequences (restriction sites) on both the gene sequence and plasmid DNA, and cutting the DNA at these sites. This creates compatible ends that can be ligated together to form a hybrid molecule. The enzyme ensures precise, targeted manipulation of DNA sequences in genetic engineering applications.


In a paper on catalytic activity of an enzyme in which section would you find detailed information on how the enzyme was prepared and handled in the experiment?

Material and method


How do you find suitable plasmid?

if you are looking for a repository grade plasmid you should search the link below but beware of what you buy through some of the more shady vendors here: importer.ec21.com/buy_leads/plasmid.html


Can genetic engineering techniques treat cystic fibrosis?

Yes, there are two similar techniques in which i am aware of.AdenovirusesFirstly adenoviruses are made harmless by interefering with a gene involved in replication. A healthy from of the CFTR gene is extracted and cut with restriction endonucleases, the same enzyme is used to cut a bacterial plasmid. The gene and plasmid are mixed together along with DNA ligase to anneal the phosphosugar framework of the DNA fragment and bacterial plasmid. The plasmid is then mixed with epithelial cells. The plasmid is then isolated and purified and places into adenoviruses. These are then placed onto the nostrils of individuals with cystic fibrosis. The viruses find their way to epithelial cells in the airways and injected their DNA. The DNA contains the functional CFTR gene, the cells can then produce fucntional CFTR proteins.LiposomesA healthy gene is extracted from a human. This gene is then inserted into a bacterial plasmid, in a similar manner as discussed above. The bacterial plasmids are then inserted into bacteria. These are allowed to grow and divide, producing large quantities of the plasmid, with the required gene. These plasmids are then extracted and coated in a lipid soluble substance. They are then put into nasal sprays and sprayed onto the nostrils of effected individuals.


How can you obtain large amounts of enzymes if the source is not sufficient for producing them?

You would need to find more sources for the enzyme.


Who are the important people you would find in the mosque?

you would find Muslim people.


Does pjhgeph-c4 have a poly-histidine tag?

where is it from? someone would have to add a his tag on purpose. There are imuno-assays available to detect a his tag. Otherwise find out if the his tag was introduced when it was cloned to a plasmid. You should have access to these papers.


How do you get the telekinesis plasmid in BioShock?

There is no teleport plasmid in any of the Bioshock games. In the Fontaine Futuristics level in Bioshock 2, there is an unstable teleport plasmid sitting on a desk. If you interact with it, it will teleport somewhere in the room. Keep following it and it will lead you to the vending expert 2 gene tonic.


Where can i find lizozim?

Lyzozyme is an enzyme found in tears, saliva, and egg whites. You can purchase lysozyme supplements from health food stores or online retailers if you are looking for a concentrated form of the enzyme.


Why would it be important to find water on the moon?

Life in space dududuudiedjx


On which plate would you expect to find bacteria most like the original non transformed E. coli colonies you initially observed?

You would expect to find it in the plate labeled LB-, because that specific plate is the control plate, meaning it has nothing added to it, like the amipicilin or pFlouroGreen plasmid.