Koch's recommendation for fixing and staining is important for microbiology even though all bacteria can be seen under the microscope, because staining and fixing is needed in order to see inside of a transparent bacteria.
Fixing the smear kills the bacteria and staining makes it more visible under the microscope.
heat fix is necessary because when we wash the slid then bacterian cell can not stay.
improper fixing
adv..... heat fixing inactivates enzymes that would normally disrupt cell morphology and structure during staining and observation. dis....... if not done well much of the specimen can be destroyed in heat fixing.
One of the disadvantages of heating fixing is that it is very expensive. Secondly, it cannot be used in capsular staining.
why are nitrogen fixing bacteria so important to other organism
If no heat fixing was done to a slide with a specimen on it, it would be rinsed off with the gram staining procedure. Heat fixing the specimen does kill specimen but it also locks it in place.
improper fixing
adv..... heat fixing inactivates enzymes that would normally disrupt cell morphology and structure during staining and observation. dis....... if not done well much of the specimen can be destroyed in heat fixing.
If no heat fixing was done to a slide with a specimen on it, it would be rinsed off with the gram staining procedure. Heat fixing the specimen does kill specimen but it also locks it in place.
One of the disadvantages of heating fixing is that it is very expensive. Secondly, it cannot be used in capsular staining.
First and foremost, the purpose of heat fixing is to drive stain into the bacterial cells, which in this case, you are staining the background, so there is not a need for heat fixing. Next, the process of heat fixing will shrink the cell by a little. This sorts of support the first reason as since there isn't the need to heat fix, then don't. By not heat-fixing, we actually see a more accurate morphology, arrangement and size of thr bacterial cell. Hope that my answers helps 😊
why are nitrogen fixing bacteria so important to other organism
fixing the stain so that the first dye which is the crystal violet will not be washed away during rinse process.
If no heat fixing was done to a slide with a specimen on it, it would be rinsed off with the gram staining procedure. Heat fixing the specimen does kill specimen but it also locks it in place.
Which is the most important and abundant carbon-fixing enzyme on earth
Heat fixing is done to kill the specimen; adhere the specimen to the slide; and alter the specimen so that they more readily accept stains.
You heat fix a slide by passing it through a blue flame a couple of times (with th cells facing up). you do this to denature any enzymes that might lyse the cells or interfere with the staining procedure. you also use it kill the organism and to adhere the organism to the slide for staining
The purpose of heat fixing the bacteria to the slide is so during the gram staining procedure the bacteria doesn't wash off. If you didn't heat fix the bacteria to the slide, it would not stay on the slide.