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Why you sterlize at 121 Celsius temp for 15 minutes in autoclave?
Wiki User
∙ 12y ago
The sterilisation temperature is defined by the spores of the Geobacillus stearothermophilus. The spores have a D-value of 1,5 min @ 121°C.
D-value means decimal reduction criteria, so it means, that after a treatment of 1,5 min (90 sec) at 121°C 90% of the bacillus spores have "died".
For your process that means a 10x 90% reduction, a 10D concept. This means after 15 min @ 121°C 99,99999999% of the spores of G. stearothermophilus are "gone".
Usual, the food industry demands commercial sterility, which is defined with a 12D concept.
The regular sterilization requirement is usually 20min @121°C (or 5min @ 135°C). Where is your 15min@121°C sterization concept used?
Cheers,
Abraxas
Wiki User
∙ 13y ago
Wiki User
∙ 12y ago121 Celsius for 15mins chosen- to kill all microbes including endospores
Temperature will not damage any items which has been under sterilization in autoclave
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2 glasses of water 1 with a temp of 18deg celsius and the other 10degrees celsius are placed in a refrigirator whose inside temperature is 7deg celsius Which of the glasses will cool first?
Assuming they're of the same size and material the 10 deg glass will reach 7 deg first, it's only got 3 deg to go.
What is the incubator temp for bacteria on agar to grow?
Depends on the type of bacteria. Mesophiles (the most common and commonly cultured) grow optimally at 37* Celsius (or around there, depending on the type). Thermophiles (heat loving bacteria) grow well around 60-80* C, and Psychrophiles (cold loving bacteria) grow around 15* Celsius.
What happens to bacteria at 100 degrees?
most bacteria thrive in heat, multiply faster. But at very high temperature, they stop growing. Some bacteria have the ability to survive at temp of more than 100 degrees Celsius. They are known as extremophiles
What would cause dry cough fatigue and low fever?
Most likely a cold (if temp is under 100) or the flu (if temp is over 100).
What is the process of the body maintaining normal internal stability called?
Homeostasis- when an organism's internal temp is unchanged, even when the external temp does change
What is the equation for changing Celsius to kelvin?
Temp. Celsius = Temp. Kelvin - 273.15
Why is silver nitrate added to plant tissue culture medium after autoclave?
silver ions will be reducted by sugar to silver metal in high temp autoclave process.
What is the formula on how to convert degree Celsius into rankine?
Rankine temp = 1.8(Celsius temp) + 491.67
What does 17 Fahrenheit equal in Celsius?
Use this formula. Temp. Fahrenheit = Temp. Celsius(1.80) + 32 so, 17 F = Temp. Celsius(1.80) + 32 ( subtract 32 from each side ) -15 = Temp. Celsius(1.80) ( divide each side by 1.80 ) -8.3 = Temperature Celsius
What is the celsius temp if the temp is 85 Fahrenheit?
about 29.4444
If the absolute temp of a is 600 k the temp in degrees celsius?
-273.15+600=326.85 degrees celsius
What is 150 degress Fahrenheit in Celsius?
65.6 degrees Celsius (I rounded the temp up, since it was 65.55...) you can calculate the Celsius temp by subtracting 32 from the Fahrenheit temp, then multiply that number by 5 and then divide the number by 9. (F temp - 32) 5/9 = Celsius
Maximum surface temperature on mars in celsius?
The surface temperature of Mars in celsius is 23 Celsius. Here are some other temperatures you might be interested in: Mercury surface temp: 427 Celsius Venus surface temp: 484 Celsius Earth surface temp: 15.0 Celsius Hope this helped :)
What is the temp temp of the inner core?
it is 6,650 degrees Celsius
If the temp is 32 degrees Fahrenheit what is the Celsius temp?
Zero
What is the celsius temp when the Fahrenheit temp is 350?
350ºF = 176.7ºC
What is Jupiter's average temp in Celsius?
-130 Degrees Celsius
