Gram stain of tissue biopsy test involves using crystal violet stain to test a sample of tissue taken from a biopsy.
The Gram stain method can be applied to almost any clinical specimen and is an excellent technique for making a general, basic identification of the type of bacteria present in the sample.
How the test is performedA sample, called a smear, from a tissue specimen is usually applied in a very thin layer to a microscope slide. The specimen is stained with crystal violet stain and undergoes additional processing before it is examined under the microscope for the presence of microorganisms. Different characteristics of the microoganisms, such as color, shape and pattern of staining, help determine the type of microorganism.
How to prepare for the testIf the biopsy is included as part of a surgical procedure, you will be asked not to eat or drink anything the night before surgery. If the biopsy is of a superficial (on the surface of the body) tissue, you may be asked not to eat or drink for several hours before the procedure.
How the test will feelHow the test feels depends on the part of the body being biopsied. There are several different methods for obtaining tissue samples. A needle may be inserted through the skin to the specific tissue. An cut (incision) through the skin into the tissue may be made, with a small piece of the specific tissue removed.
A biopsy may also be taken from inside the body by an instrument that helps the doctor see inside the body, such as an endoscope or cystoscope. Some form of anesthetic is usually given so there is little or no pain. Pressure and occasional mild pain may be felt during a biopsy.
Why the test is performedThe test is performed when an infection of a body tissue is suspected.
Normal ValuesThe presence or type of organisms depends on the particular tissue being biopsied. Some tissues in the body are sterile, such as the brain, whereas other tissues normally contain organisms.
Note: Normal value ranges may vary slightly among different laboratories. Talk to your doctor about the meaning of your specific test results.
What abnormal results meanAbnormal results usually indicate an infection in the tissue site. Further tests, such as culturing the biopsied material, are frequently needed to identify the specific type of organism involved.
What the risks areThe only risks are those associated with obtaining a tissue biopsy and may include bleeding or infection.
No, acid-fast bacteria do not stain gram-negative when subjected to the gram stain.
Gram stain
The counter or secondary stain used in the Gram stain procedure is safranin.
Yogurt contains mostly lactic acid bacteria, which are Gram-positive bacteria. This means they will stain purple under a Gram stain.
In the flagella stain, all cells appear purple due to the basic dyes used to stain the flagella. This uniform coloration helps visualize the flagella structure under the microscope. In contrast, the Gram stain uses a series of dyes to differentiate between Gram-positive (purple) and Gram-negative (pink) cells based on their cell wall composition.
No, acid-fast bacteria do not stain gram-negative when subjected to the gram stain.
Gram stain
Gram Negative
Protists are often stained using a silver stain, not a Gram stain.
The Gram stain is used for bacteria and not for viruses.
The biopsy tissue sample is called a "biopsy specimen." This specimen is obtained from a suspicious area of tissue during a biopsy procedure and is then examined microscopically to diagnose diseases, including cancer. The analysis of the biopsy specimen helps determine the nature of the tissue, its characteristics, and any potential abnormalities.
Enterobacter cloacae is a Gram-negative bacterium. It will stain pink or red in a Gram stain procedure.
It is a biopsy
That is the correct spelling of "biopsy" (tissue sample).
Bacteria stain either gram-positive or gram-negative based on the presence or absence of a cell wall. Viruses do not pick up a gram stain.
The counter or secondary stain used in the Gram stain procedure is safranin.
The medical term for examination of tissue under a microscope is called- Histology