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Technically it is not a substance, but the DNA itself. Let me explain. When the insulin gene is cut out of a regular strand, it is done through a DNA ligase (a cutting enzyme). The SAME ligase is used to cut the bacterial loop of DNA. When ligase cuts DNA sticky ends are left. These sticky ends are, as they say, sticky, and will readily join to new bases with the corresponding (complementary) base pair sequence. As the same ligase is used, the corresponding base sequence is inside the bacterial DNA, so they should connect together.
What else can I help you with?
How recombinant DNA has mass produced insulin?
By rDNA technology, the gene of interest can be transformed in to a lab organism,say bacteria; and by expressing that gene, large production of insulin or any other factor is possible. This can be tested for its activity after purification of the protein from the crude bacterial lysate.
Which best describes how scientists found the hunan gene that makes insulin?
Scientists found the human gene that makes insulin by using recombinant DNA technology to clone and sequence the gene. They identified the gene by comparing it to the insulin genes of other species and then inserting the human gene into bacteria to produce insulin for medical use.
What is One difference between the gene that codes for insulin and the gene that codes for testosterone in humans?
The gene of insulin has a different sequence of molecular bases than the gene of testosterone.
A certain mutant bacterial cell cannot produce substance x. the mutation was most likely the result of a change in the?
The mutation in the bacterial cell most likely occurred in the gene responsible for producing substance X. This change in the gene's sequence could have been caused by various factors like spontaneous errors during DNA replication, exposure to mutagens, or genetic recombination events.
Which type of DNA technology is used to cause bacteria to produce human insulin?
The type of DNA technology used to cause bacteria to produce human insulin is recombinant DNA technology. In this process, the gene for human insulin is inserted into the genome of a bacterium, such as Escherichia coli (E. coli), using techniques such as restriction enzymes and ligase enzymes. Once the gene is inserted, the bacterium is then able to produce human insulin, which can be purified and used for medical purposes. This technology has revolutionized the production of insulin, making it more accessible and affordable for people with diabetes. Recombinant DNA technology has also been used to produce many other human proteins, such as growth hormone and blood clotting factors, with great success.
How recombinant DNA has mass produced insulin?
By rDNA technology, the gene of interest can be transformed in to a lab organism,say bacteria; and by expressing that gene, large production of insulin or any other factor is possible. This can be tested for its activity after purification of the protein from the crude bacterial lysate.
How does a human insulin genes become part of a plasmid?
1. Scientists remove plasmids, small rings of DNA, from bacterial cells. 2. An enzyme cuts open the plasmid DNA. The same enzyme removes the human insulin gene from its chromosome. 3. The human insulin gene attaches the open ends of the plasmid to form a closed ring. 4. Some bacterial cells take up the plasmids that have the insulin gene. 5. When cells reproduce, the news cells will contain copies of the engineered plasmid. The foreign gene directs the cell to produce human insulin.
How can a large volume of protein e.g. insulin be produced from a single bacterium?
The gene for insulin can be inserted into the bacterial chromosome. The bacteria is then left to multiply normally, which thus produces many copies of the gene and lots of insulin. This is how they produce the insulin used by people who have diabetes.
Which molecule from humans is inserted into bacteria to cause them to produce insulin?
The human gene that codes for insulin is inserted into bacteria to produce insulin. The gene is typically inserted into a plasmid vector, which allows the bacteria to express the human insulin gene and produce insulin. This technique is used in biotechnology to create recombinant bacteria that can produce insulin for medical use.
How is bacteria used to make insulin?
Human plasmids introduced into the bacteria stimulate insulin production. A special enzyme is used to cut out the insulin gene from a human cell. It is attached to a bacterial chromosome which is also split open by an enzyme. The gene is then transferred into a bacterial cell. The gene makes the bacterial cell produce insulin.
What is the process of making human insulin with bacteria?
The process involves inserting the human gene for insulin into a bacterial plasmid, which acts as a vector. The bacteria then replicates the gene and produces insulin protein. The protein is harvested, purified, and formulated into insulin for medical use.
Which best describes how scientists found the hunan gene that makes insulin?
Scientists found the human gene that makes insulin by using recombinant DNA technology to clone and sequence the gene. They identified the gene by comparing it to the insulin genes of other species and then inserting the human gene into bacteria to produce insulin for medical use.
What does the bacterial cell reproduce that the human gene codes for?
the bacterial cell reproduces the bacterial chromosome that the human gene codes for.
What is One difference between the gene that codes for insulin and the gene that codes for testosterone in humans?
The gene of insulin has a different sequence of molecular bases than the gene of testosterone.
When pharmaceutical companies use recombinant bacteria to make insulin what do they insert the insulin gene into?
The human insulin gene, which is located on the top of the short arm of chromosome 11 in human DNA, is cut from the DNA strand using restriction enzymes (genetics scissors). A plasmid (floating circular disks of DNA in bacteria) is extracted from a bacteria and cut open with another restriction enzyme, and the gene for human insulin is taken up by the plasmid. Another enzyme, ligase, is used to permanently seal the exposed nucleotides (ends of the DNA strands) together (like genetic glue). the plasmid is then put back into the bacterial cell, and the bacteria will then manufacture insulin. its offspring will also have the genetic data for human insulin.
Why is the bacterium now able to make insulin?
The bacterium has been genetically modified to contain the human insulin gene. This gene allows the bacterium to produce insulin when it is transformed with the gene and given the appropriate conditions for protein synthesis.
Do skin cells copy and translate an insulin gene into insulin protein?
Insulin is not produced by skin cells.
