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Barfoed's test is used for detecting the presence of monosaccharides.
It is based on the reduction of copper(II) (as acetate) to copper(I) oxide (Cu2O), which forms a brick-red precipitate. (Disaccharides may also react, but the reaction is much slower.)
Composition:
Barfoed's reagent consists of a 0.33 molar solution of neutral copper acetate in 1% acetic acid solution. The reagent does not keep well and it is therefore advisable to make it up when it is actually required.
The aldehyde group of the monosaccharide which normally forms a cyclic hemiacetal is oxidized to the carboxylate. A number of other substances, including sodium chloride may interfere.
The test is similar to the reaction of Fehling's solution to aldehydes or Benedict's test.
There is no difference for a solution to be tested by Benedict`s or by Barfoed`s test, as both detect the presence of reducing sugars. Barfoed`s test is the least known of these three.
Wiki User
∙ 14y ago
Wiki User
∙ 6y agoBarfoed reagent contain copper actete in acetic acid. It is applied to monosaccharides.
Benedict reagent contain copper sulfate, sodium carbonate and sodium citrate. It is applied to more substances.
Wiki User
∙ 7y agoCopper sulfate is used in Fehling test and and copper acetate is used in Barfoed test.
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Which is more sensitive test fehlings test or Benedicts test?
benedicts test
What test show hydrolysis of proteins?
benedicts test
Are substance considered reducing fehlings test also the substance reducing in benedicts test?
yes.
What would the result of crackers in benedicts test?
Turns blue black
Will hydrolysed dextrine give positive response to Benedict test?
Yes, it should. Benedicts test will be positive for reducing sugars, and since glucose is such a sugar, and would be a product of dextrin hydrolysis, you should get a positive result with Benedicts reagent.
What is barfoed test?
The Barfoeds test will allow us to make a distinction between reducing monosaccharides and reducing disaccharides. If the Red tetrazolium test is done first and is positive for a reducing sugar, then the Barfoeds test will tell us if it is mono or di.
Which is more sensitive test fehlings test or Benedicts test?
benedicts test
What test show hydrolysis of proteins?
benedicts test
Do eggs test positive in benedicts test?
yes
What are the Benedicts test results for Gatorade?
penis
What is the test for sugar using Benedicts solution?
prosrdure of benidect solution test
What is the difference between diagnostic test and proficiency test?
what is the difference between proffiency and diagnostic test
What is the difference between proficiency test and diagnostic test?
what is the difference between proffiency and diagnostic test
Are substance considered reducing fehlings test also the substance reducing in benedicts test?
yes.
What would the result of crackers in benedicts test?
Turns blue black
What is the difference between a test and a demonstration?
The difference between a test and a demonstration is that a test is to be taken and answered and a demonstration is to be demonstrated and be told to the class
Will hydrolysed dextrine give positive response to Benedict test?
Yes, it should. Benedicts test will be positive for reducing sugars, and since glucose is such a sugar, and would be a product of dextrin hydrolysis, you should get a positive result with Benedicts reagent.
