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Hopkins cole reagent

Updated: 8/11/2023
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9y ago

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3.0 g Mg powder or thin ribbon cut in small pieces, cover with distilled water and cool in an ice bath. Slowly add with stirring 75 mL cold, saturated oxalic acid solution (about 8 g oxalic acid in 75 mL water). Filter, and 7.5 mL glacial acetic acid to the filtrate. Top up to 150 mL with distilled water.

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14y ago
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9y ago

Benedict-Hopkins-Cole reagent is magnesium glyoxalate that is produced from a combination of magnesium and oxalic acid. It is utilized to test proteins for tryptophan.

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Related questions

When was Albert Cole Hopkins born?

Albert Cole Hopkins was born in 1837.


When did Albert Cole Hopkins die?

Albert Cole Hopkins died in 1911.


What chemical grouping is responsible for a positive Hopkins-Cole test?

Tryptophan


What is the Hopkins-Cole test?

The hopkins-cole test is used to determine the presence of the amino acid tryptophan. Tryptophan has an indole nucleus which is responsible for the violet ring found at the junction between the two layers.


What is the role concentrated h2SO4 in the hopkins-Cole test?

gdg


What is the role of concentrated H2SO4 in Hopkins-cole test?

to hydrolysed the trytophan


What is the Hopkins Cole test?

The hopkins-cole test is used to determine the presence of the amino acid tryptophan. Tryptophan has an indole nucleus which is responsible for the violet ring found at the junction between the two layers.


Will tyrosine test positive in Hopkins-Cole test?

Yes, it is possible (color test).


What is the amino acid responsible for the Hopkins-cole reaction?

The amino acid responsible for the Hopkins-Cole reaction is the tryptophan because of its indole ring that in the reaction forms a violet color upon treatment of the sample with glyoxylic acid and sulfuric acid.


What is the color produced at the point of contact of the two liquids in Hopkins-Cole reaction?

This color is purple.


What is the name of the amino acid responsible for Hopkins-cole reaction test what is the formula of this amino acid?

Tryptophan.


What is Hopkins's test?

The hopkins-cole test is used to determine the presence of the amino acid tryptophan. Tryptophan has an indole nucleus which is responsible for the violet ring found at the junction between the two layers.