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3.0 g Mg powder or thin ribbon cut in small pieces, cover with distilled water and cool in an ice bath. Slowly add with stirring 75 mL cold, saturated oxalic acid solution (about 8 g oxalic acid in 75 mL water). Filter, and 7.5 mL glacial acetic acid to the filtrate. Top up to 150 mL with distilled water.
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What is the role concentrated h2SO4 in the hopkins-Cole test?
Concentrated H2SO4 is used in the Hopkins-Cole test to dehydrate carbohydrates present in the sample, forming furfural derivatives that react with the reagent to produce a color change. This color change is then used to detect the presence of pentoses, specifically ribose and deoxyribose sugars.
What is the Hopkins-Cole test?
The hopkins-cole test is used to determine the presence of the amino acid tryptophan. Tryptophan has an indole nucleus which is responsible for the violet ring found at the junction between the two layers.
What is the role of concentrated H2SO4 in Hopkins-cole test?
Concentrated H2SO4 is used in the Hopkins-Cole test to dehydrate the carbohydrates present in the sample, converting them into furfural derivatives. These derivatives then react with aniline to produce a colored product, allowing for the detection of the presence of pentoses and hexoses in the sample.
What is color of Hopkins-cole test?
The Hopkins-Cole test is a test for detecting the presence of reducing sugars, such as glucose, in a sample. The color change observed in the test varies depending on the specific reagents used, but it typically involves a color change from blue to green or yellow in the presence of reducing sugars.
What is the positive result in the hopkin's- cole test for proteins?
a purple /violet ring is formed at the junction .. that's what i get in our experiment.. and its correct..:)
When was Albert Cole Hopkins born?
Albert Cole Hopkins was born in 1837.
When did Albert Cole Hopkins die?
Albert Cole Hopkins died in 1911.
What is the role concentrated h2SO4 in the hopkins-Cole test?
Concentrated H2SO4 is used in the Hopkins-Cole test to dehydrate carbohydrates present in the sample, forming furfural derivatives that react with the reagent to produce a color change. This color change is then used to detect the presence of pentoses, specifically ribose and deoxyribose sugars.
What chemical grouping is responsible for a positive Hopkins-Cole test?
Tryptophan
What is the name of the amino acid responsible for Hopkins-cole reaction test what is the formula of this amino acid?
The amino acid responsible for the Hopkins-Cole reaction test is tryptophan. Its formula is C11H12N2O2.
What is the Hopkins Cole test?
The hopkins-cole test is used to determine the presence of the amino acid tryptophan. Tryptophan has an indole nucleus which is responsible for the violet ring found at the junction between the two layers.
What are the principles in Hopkins Cole precipitation test for protein?
The Hopkins-Cole precipitation test is based on the principle that certain proteins, particularly tryptophan-containing proteins, react with the reagent glyoxylic acid in the presence of concentrated sulfuric acid to produce a colored complex. When the test solution is layered carefully over the sulfuric acid, a characteristic purple ring forms at the interface if the protein is present. This reaction is specific and sensitive, making it useful for detecting proteins in a sample. The intensity of the color can also be correlated with the concentration of the protein.
What is the amino acid responsible for the Hopkins-cole reaction?
The amino acid responsible for the Hopkins-Cole reaction is the tryptophan because of its indole ring that in the reaction forms a violet color upon treatment of the sample with glyoxylic acid and sulfuric acid.
What is the Hopkins-Cole test?
The hopkins-cole test is used to determine the presence of the amino acid tryptophan. Tryptophan has an indole nucleus which is responsible for the violet ring found at the junction between the two layers.
What is the color produced at the point of contact of the two liquids in Hopkins-Cole reaction?
This color is purple.
Will tyrosine test positive in Hopkins-Cole test?
Yes, tyrosine will test positive in the Hopkins-Cole test. This test is used to detect the presence of phenylketonuria (PKU) by identifying the abnormal accumulation of phenylalanine and tyrosine in the blood.
What is the role of concentrated H2SO4 in Hopkins-cole test?
Concentrated H2SO4 is used in the Hopkins-Cole test to dehydrate the carbohydrates present in the sample, converting them into furfural derivatives. These derivatives then react with aniline to produce a colored product, allowing for the detection of the presence of pentoses and hexoses in the sample.
