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To make a buffer solution, mix a weak acid and its conjugate base or a weak base and its conjugate acid in a specific ratio. This helps maintain a stable pH level when small amounts of acid or base are added.

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4mo ago

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Can vinegar be used to make a buffer solution?

No, vinegar cannot be used to make a buffer solution. A buffer solution typically consists of a weak acid and its conjugate base, such as acetic acid and sodium acetate. Vinegar is a dilute solution of acetic acid only and lacks the necessary components to act as a buffer.


A solution that is resistant to change in pH?

A buffer solution is resistant to changes in pH because it contains a weak acid and its conjugate base, which can react with added acid or base to maintain a relatively constant pH. Buffers are commonly used in biochemical and chemical systems to prevent drastic changes in pH levels.


What can be mixed in solution with H2CO3 to make a buffer?

A weak acid or its conjugate base can be mixed in solution with H2CO3 to make a buffer. Suitable options could be sodium bicarbonate (NaHCO3) or a weak acid like acetic acid (CH3COOH).


How do you make 1m kh2po4 buffer?

Make Solution A by dissolving 174.18g of K2HPO4 in 1L of dH2O. Make solution B by dissolving 136g of KH2PO4 in 1L of dH2O. now mix solution A and B and finally adjust pH of your buffer.


How do you convert 1x buffer to 10x buffer?

The stocks are commonly labeled as X factors such as 10X, 5X, 100X etc. X-factor indicates that the solution is concentrated and must be diluted usually with water to 1X concentration for use. For eg: - A 100X concentrated solution should be diluted to 100 fold. to convert 1X to 10X take one ml of 1x buffer in a measuring cylinder and dilute it to make it 10 ml. its now 10x buffer.

Related Questions

Can be mixed in a solution with H2CO3 to make a buffer?

H2CO3 is not used as buffer.


Can vinegar be used to make a buffer solution?

No, vinegar cannot be used to make a buffer solution. A buffer solution typically consists of a weak acid and its conjugate base, such as acetic acid and sodium acetate. Vinegar is a dilute solution of acetic acid only and lacks the necessary components to act as a buffer.


How do you make buffer solution pH10?

See the link below.


How do you make this solution?

I don't know how to make the solution below. Low salt buffer: 10 mM phosphate buffer, 10 mM NaCl, pH 7.4. Could you tell me the method in detail?


A solution that is resistant to change in pH?

A buffer solution is resistant to changes in pH because it contains a weak acid and its conjugate base, which can react with added acid or base to maintain a relatively constant pH. Buffers are commonly used in biochemical and chemical systems to prevent drastic changes in pH levels.


What can be mixed in solution with H2CO3 to make a buffer?

A weak acid or its conjugate base can be mixed in solution with H2CO3 to make a buffer. Suitable options could be sodium bicarbonate (NaHCO3) or a weak acid like acetic acid (CH3COOH).


How do you make 1m kh2po4 buffer?

Make Solution A by dissolving 174.18g of K2HPO4 in 1L of dH2O. Make solution B by dissolving 136g of KH2PO4 in 1L of dH2O. now mix solution A and B and finally adjust pH of your buffer.


How do you make urease solution?

To make a urease solution, simply dissolve urease enzyme powder in an appropriate buffer solution of your choice, such as phosphate buffer at the desired pH. The concentration of urease in the solution will depend on the specific experiment or assay you are conducting, so adjust the concentration as needed. Remember to keep the solution cold and handle the enzyme with care to maintain its activity.


What is TNE buffer?

tris, EDTA (TE solution) and NaCl, TNE buffer is a buffer solution used in molecular biology, especially for DNA and RNA


How can I make lysis buffer?

To make lysis buffer, mix a detergent like SDS or Triton X-100 with a buffer solution like Tris-HCl. Adjust the pH to around 7.4 and add protease inhibitors if needed. This solution helps break open cells and release their contents for further analysis.


What is the purpose of buffer?

Buffer Resist and Maintains the PH of the solution if there change in the environment of the solution.


When a base is added to a buffer solution the buffer will?

donate H+ ions