How do you get calibration curve for uv-visible spectroscopy?

Answer 1

A spectrometer is normally a system that detects changes in the way light passes thru a sample. These machines are utilized largely inside research labs in Universities, private companies, and professional industries. There are hundreds of different types of these machines. However all of them work the same way.

Do I really need to calibrate a UV VIS spectrophotometer each time I use it?

The short answer to this question is no, you don't have to calibrate each and every time you use your machine. However, making sure the spectrophotometer or spectrometer is calibrated correctly is of the utmost importance. If the machine is giving incorrect readings, then researchers and scientists will just be wasting precious time and money. Also calibrating will tell you if your light source(s) are getting old and need to be replaced. Difficulty: Super Easy

InstructionsTools you'll need:

• UV VIS Spectrophotometer Calibration Standards
• Spectrophotometer or Spectrometer
• Certificate of Calibration

Step 1: Turn on the spectrometer or spectrophotometer and let it warm up for at least 15 minutes. Check your manual to see if your machine has a longer or shorter warm-up period.

Step 2: Choose the wavelength that you need to calibrate.

Step 3: Select the appropriate calibration filter thickness. This can be found in the owner's manual in the "How to Calibrate A UV VIS Spectrophotometer" Section.

Step 4: Inspect the calibration filter to ensure no dust, oils, or debris is on the optical surfaces.

Step 5: Carefully load the filter into the cuvette holder. Close the lid and wait for the measurement.

Step 6: Compare the results to what is specified on the manufacturers Certificate of Calibration.

Step 7: If the numbers match within +/- 5%, your spectrophotometer/spectrometer is calibrated correctly.

Step 8: If the numbers are not within the +/-5% tolerance, then adjustments need to be made to your machine.