How do you prepare 0.1 M citrate buffer pH 4.5?

Answer 1

Preparation

0.1MCitrate Buffer

1. Add approximately 50 mL of distilled water to a

100ml volumetric flask on a magnetic stirrer.

2. Add and dissolve 1.4705gm of Sodium citrate.

3. Then with the help of Citric Acid maintain the pH of the solution upto 4.5.

Answer 2

You have to simply dissolve streptozotocin (STZ) in the freshly prepared citrate buffer pH 4.5. But first you have to calculate the amount of STZ and the volume of citrate buffer required. Here's an example for a rat weighing 150 gms. STZgiven at 45 mg/kg of body weight. So for 150 gms you need 6.75 mg of STZ. Now you need to inject this in the abdominal cavity of rat so prefer to use minimum volume of buffer. So here, we'll dissolve 9 mg of STZ in 1 ml of Citrate buffer pH 4.5 and inject 0.75 ml of this solution into rat. Citrate buffer as in British Pharmacopeia It would be better if STZ is dissolved just before use.

Answer 3

To make one liter of buffer, weigh out point one mole of citric acid. Dissolve it in about six hundred milliliters of water. Adjust the pH with six M NaOH until the pH is four point five. Bring the volume of the solution to one liter with water.

Answer 4

To make a citrate buffer Mix 21 g citric acid in 1 L distilled water, and 29.4 g sodium citrate in 1 L distilled water. If you only have citric acid, mix 2.1 g in just under 1 L distilled water. You can adjust the PH level by adding 1M sodium hydroxide while gently stirring the solution using a magnetic stirrer.

Answer 5

To prepare 0.1 M citrate buffer pH 4.5, dissolve the appropriate amount of citric acid (C6H8O7) and disodium hydrogen citrate (Na2C6H5O7) in distilled water. Adjust the pH to 4.5 using NaOH or HCl. Perform a final check of the pH before use.