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Bromothymol Blue (BTB)

Bromothymol blue is an indicator, a substance that changes color as the pH of a solution changes. Bromothymol blue is yellow in acidic solutions and blue in basic solutions.

  • BTB can be prepared by mixing 0.1 g (a pinch) of bromothymol blue powder in 10 mL of a 4% solution of sodium hydroxide.
  • Add 20 mL of alcohol and dilute to 1 L with distilled water.
  • The solution should be deep blue.
  • If it is green, add sodium hydroxide solution drop by drop until the solution turns blue.
  • Bromothymol blue can be purchased from scientific supply houses.

CAUTION: Sodium hydroxide is corrosive; the BTB solution should be prepared in a laboratory. BTB stains hands and clothes. Have students wear goggles and follow safety precautions when using BTB solution.

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Thymol blue

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Q: How do you prepare thymol blue indicator?
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Related questions

In which solution will thymol blue indicator appears blue?

KOH


Why is thymol blue used as the indicator for titration of acetic acid and naoh?

An indicator that changes its colour around pH value 5 should be used in this titration. Alternatively, instead of Thymol Blue, Methyl Orange or Methyl Red may be used.


What indicator's could be used to produce a color change at both PH 2 and PH?

thymol blue,cresol red,and universal indicatior


What colour would thymol blue go if hydrochloric acid was added?

Thymol blue will be yellow at pH < 8


What is the absorbance value for thymol?

thymol blue 436, 545 and 595 nm


The name of the plant which universal indicator is made of?

From wikipedia: A universal indicator is typically composed of water, methanol, propan-1-ol, phenolphthalein sodium salt, methyl red, bromothymol blue monosodium salt, and thymol blue monosodium salt


What does universal indicator contain?

Water, propan-1-ol, phenolphthalein sodium salt, sodium hydroxide, methyl red, bromothymol blue monosodium salt, thymol blue monosodium salt.


Why does the typical base indicator exhibit its color change over a range of about 2 PH units?

The usual range of the color change on the indicator is between 1.5-2 pH units because of the sensitivity of the indicators. The universal indicator is used in most test.


What substance is used as an acid-base indicator?

Phenolphthalein, Metyl Orange, Methyl Red, Thymol Blue and Bromothymol Blue are some of the commonly used acid-base indicators. The property of such indicators should be the capability of showing a distinct chemical change with respect to a particular pH value.


Universal indicator is a mixture of what?

Universal indicators can be mixed with different substances. Universal indicators usually contain water, salt, propan-1-ol, sodium hydroxide, monosodium, phenolphthalein sodium salt, methyl red, bromothymol blue, and thymol blue monosodium salt


What is simmons citrate agar?

Simmons citrate agar is a differential agar used to determine if a sample bacteria can utilize citrate as its only carbon source. The agar is initially a green color due to the bromo thymol blue pH indicator in it. If a bacteria uses the citrate, the by-products are ammonia and ammonium hydroxide both of which will alkalize the agar and increase the pH to the point of changing the indicator's color to blue, so the whole agar turns from green to blue.


What is the chemical formula of trypan blue?

what is the chemical formula for thymol blue