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What is the absorbance value for tartrazine?
The absorbance value for tartrazine will depend on the specific wavelength at which it is measured. Tartrazine typically absorbs light most strongly in the visible spectrum, around 425-430 nm. To determine the exact absorbance value, you would need to measure the absorbance of a known concentration of tartrazine at this wavelength using a spectrophotometer.
Why are the absorbance values obtained from the conversion chart rather than the absorbance scale on the spectronic 20?
because that chart gives a more accurate value than the absorbance scale on the specthometor
What does acid do to thymol blue?
Acid turns thymol blue from blue to yellow. This color change is due to the change in the chemical structure of thymol blue in response to the acidic conditions.
How does one dissolve thymol in water?
Thymol is not very soluble in water, but you can enhance its solubility by using a solvent like ethanol or propylene glycol and then adding it to water. Alternatively, you can create a thymol suspension by using a surfactant or emulsifier to disperse thymol particles in water.
What does the value of absorbance on a spectrophotometer mean?
Absorbance on a spectrophotometer is a measure of the amount of light absorbed by a sample at a specific wavelength. It provides information on the concentration of a substance in the sample since absorbance is directly proportional to concentration according to the Beer-Lambert law. A higher absorbance indicates greater absorption of light, which can be used to quantify the concentration of the absorbing species in the sample.
What has the author John Rupert Merritt written?
John Rupert Merritt has written: 'Some thymol derivatives of possible medicinal value' -- subject(s): Thymol
What is the absorbance value for tartrazine?
The absorbance value for tartrazine will depend on the specific wavelength at which it is measured. Tartrazine typically absorbs light most strongly in the visible spectrum, around 425-430 nm. To determine the exact absorbance value, you would need to measure the absorbance of a known concentration of tartrazine at this wavelength using a spectrophotometer.
Why are the absorbance values obtained from the conversion chart rather than the absorbance scale on the spectronic 20?
because that chart gives a more accurate value than the absorbance scale on the specthometor
True or false As the concentration of a colored solution increases the absorbance value decreases?
Absorbance rises with concentration because there is more material for a given frequency of light to be absorbed in. Your statement is false.
What is the relationship between the optical density (OD) and absorbance in spectrophotometry?
In spectrophotometry, optical density (OD) and absorbance are directly related. As the OD increases, the absorbance also increases. This means that a higher OD value indicates a higher absorbance of light by the sample being measured.
What does a higher absorbance value indicate in a spectrophotometry experiment?
A higher absorbance value in a spectrophotometry experiment indicates that more light is being absorbed by the sample, suggesting a higher concentration of the substance being measured.
Why the value of absorbance is always between the 0-2?
It's over 9000.
Common name of thymol iodide?
Thymol iodide is commonly known as iodoform.
How can the calibration curve of absorbance versus concentration be used to determine the concentration of a substance in a sample?
The calibration curve of absorbance versus concentration can be used to determine the concentration of a substance in a sample by measuring the absorbance of the sample and comparing it to the absorbance values on the calibration curve. By finding the corresponding concentration value on the curve, the concentration of the substance in the sample can be determined accurately.
What does acid do to thymol blue?
Acid turns thymol blue from blue to yellow. This color change is due to the change in the chemical structure of thymol blue in response to the acidic conditions.
How does one dissolve thymol in water?
Thymol is not very soluble in water, but you can enhance its solubility by using a solvent like ethanol or propylene glycol and then adding it to water. Alternatively, you can create a thymol suspension by using a surfactant or emulsifier to disperse thymol particles in water.
Why should an absorbance level not exceed a value of 1?
An absorbance value exceeding 1 can indicate that the sample is too concentrated, leading to inaccuracies in the measurement due to light scattering or saturation of the detector. To ensure accurate readings and avoid errors, it is recommended to dilute the sample to bring the absorbance within the linear range of the detector.
