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427nm plus or minus 3 nm.

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12y ago

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Why are the absorbance values obtained from the conversion chart rather than the absorbance scale on the spectronic 20?

because that chart gives a more accurate value than the absorbance scale on the specthometor


True or false As the concentration of a colored solution increases the absorbance value decreases?

Absorbance rises with concentration because there is more material for a given frequency of light to be absorbed in. Your statement is false.


What is the relationship between the optical density (OD) and absorbance in spectrophotometry?

In spectrophotometry, optical density (OD) and absorbance are directly related. As the OD increases, the absorbance also increases. This means that a higher OD value indicates a higher absorbance of light by the sample being measured.


What does a higher absorbance value indicate in a spectrophotometry experiment?

A higher absorbance value in a spectrophotometry experiment indicates that more light is being absorbed by the sample, suggesting a higher concentration of the substance being measured.


What is the absorbance value for thymol?

thymol blue 436, 545 and 595 nm


Why the value of absorbance is always between the 0-2?

It's over 9000.


What food colouring agents is least stable is it Tartrazine orSunset Yellow or Capsanthin or Erythrosineor Amaranth?

tartrazine


How can the calibration curve of absorbance versus concentration be used to determine the concentration of a substance in a sample?

The calibration curve of absorbance versus concentration can be used to determine the concentration of a substance in a sample by measuring the absorbance of the sample and comparing it to the absorbance values on the calibration curve. By finding the corresponding concentration value on the curve, the concentration of the substance in the sample can be determined accurately.


Why should an absorbance level not exceed a value of 1?

An absorbance value exceeding 1 can indicate that the sample is too concentrated, leading to inaccuracies in the measurement due to light scattering or saturation of the detector. To ensure accurate readings and avoid errors, it is recommended to dilute the sample to bring the absorbance within the linear range of the detector.


What does the value of absorbance on a spectrophotometer mean?

Absorbance on a spectrophotometer is a measure of the amount of light absorbed by a sample at a specific wavelength. It provides information on the concentration of a substance in the sample since absorbance is directly proportional to concentration according to the Beer-Lambert law. A higher absorbance indicates greater absorption of light, which can be used to quantify the concentration of the absorbing species in the sample.


Is absorbance considered a continuous variable?

Yes, absorbance is considered a continuous variable because it can take on any value within a range, rather than being limited to specific discrete values.


Is tartrazine tasteless?

No, tartrazine is not tasteless. It is a synthetic dye often used in food and beverages to provide a yellow or orange color. Some people may be sensitive to the taste of tartrazine, describing it as slightly bitter or metallic.