I have not tried that one but it looks ok
http://www.thelabrat.com/protocols/SchiffsReagent.shtml
Peter Skalinski
SFS Seoul
Formaldehyde Schiff's reagent: It is a solution of formaldehyde and sulfuric acid, used to detect the presence of aldehydes. The equation involves the formation of a colored compound with aldehydes. Benzaldehyde Schiff's reagent: Benzaldehyde can act as a Schiff's base with primary amines to form imines. The reaction results in the formation of a colored compound. Acetone Schiff's reagent: Acetone can react with a primary amine to form a Schiff's base, leading to the formation of a colored compound.
Brady's reagent is 2,4-Dinitrophenylhydrazine, its synthesised via aromatic nucleophilic substitution of 1-chloro-2,4-dinitrobenzene and hydrazine (in form of hydrazine sulphate). Its an SN2 reaction i believe. Not too sure about the mechanism yet =\
Hinsberg's reagent is benzenesulphonyl chloride. Prepared by chlorosuk
Baljet reagent is typically prepared by dissolving 2.5g of sodium nitrate and 2.5g of bismuth subnitrate in 50ml of distilled water. This solution is then added dropwise to 100ml of concentrated sulfuric acid with stirring. It is important to handle the reagents with care due to the corrosive nature of sulfuric acid.
molisch reagent is prepared by dissolving 5g of alpha napthol in 100 ml alcohol
The molecular formula of Schiff reagent is C20H15N3. It is a chemical reagent used for detecting the presence of aldehydes or ketones in organic compounds.
Formaldehyde Schiff's reagent: It is a solution of formaldehyde and sulfuric acid, used to detect the presence of aldehydes. The equation involves the formation of a colored compound with aldehydes. Benzaldehyde Schiff's reagent: Benzaldehyde can act as a Schiff's base with primary amines to form imines. The reaction results in the formation of a colored compound. Acetone Schiff's reagent: Acetone can react with a primary amine to form a Schiff's base, leading to the formation of a colored compound.
To decolorize Schiff's reagent, you can add a reducing agent like sodium metabisulfite or sodium bisulfite dropwise until the color disappears. This process helps to reverse the original color change caused by the reagent reacting with aldehydes or other carbonyl compounds.
Benzidine reagent should be freshly prepared to ensure accuracy and reliability of the results. It is prone to oxidation and degradation over time, which can lead to false positive or negative test outcomes. Freshly prepared benzidine reagent will provide the most accurate and consistent results in tests.
The Schiff's reagent, which is a mixture of fuchsin-sulfurous acid, is used to detect the presence of aldehydes or compounds with free aldehyde or ketone groups in a sample. When the reagent reacts with the aldehyde group, it forms a magenta-colored complex due to the reduction of the fuchsin dye. This color change is the basis of the Schiff's test, indicating the presence of aldehydes in the sample.
Brady's reagent is 2,4-Dinitrophenylhydrazine, its synthesised via aromatic nucleophilic substitution of 1-chloro-2,4-dinitrobenzene and hydrazine (in form of hydrazine sulphate). Its an SN2 reaction i believe. Not too sure about the mechanism yet =\
Ninhydrin reagent is prepared by dissolving ninhydrin powder in a solvent such as ethanol or acetone. The solution is typically heated gently to aid dissolution. It is important to prepare fresh ninhydrin reagent before use to ensure its effectiveness in detecting amino acids.
Hinsberg's reagent is benzenesulphonyl chloride. Prepared by chlorosuk
Anthrone reagent is prepared in concentrated sulfuric acid. Adding reagents to acid causes immediate dissociation of the reagent and releases heat. If your solution weren't cold, it could generate enough heat to blow out of the flask or bottle.
Chromic acid reagent is prepared by dissolving chromium trioxide in water. It should be handled with caution as it is a strong oxidizing agent and can be corrosive. It is commonly used in laboratory settings for oxidation reactions.
(Wagner's reagent is used to test for alkaloids). To prepare Wagner's reagent; Dissolve 2g of iodine and 6g of KI in 100ml of water. -Atolani Olubunmi, Nigeria
Baljet reagent is typically prepared by dissolving 2.5g of sodium nitrate and 2.5g of bismuth subnitrate in 50ml of distilled water. This solution is then added dropwise to 100ml of concentrated sulfuric acid with stirring. It is important to handle the reagents with care due to the corrosive nature of sulfuric acid.