0
1 mole of EDTA will chelate with 1 mole of Ca2+ ions or 1 mole of Fe2+ or Fe3+ ions.
Wiki User
∙ 11y ago
Add your answer:
Earn +20 pts
How do you prepare 5mM EDTA of 100ml solution?
5mM = 0.005 moles 100 mL = 0.1 Liters Molarity = moles of solute/Liters of solution 0.005 M EDTA = X moles/0.1 Liters = 0.0005 moles EDTA =_____________ Now, look up the molecular formula for EDTA and find how many grams needed to add to your 100 mL.
How much quantity of EDTA dissolved in 1000ml to get 0.01 m solution of EDTA?
1 mole EDTA has 4 equivalents. 0.01 N EDTA = 0.01 equivalents/liter, so you need 0.01/4 = 0.0025 moles EDTA/liter.
Why is magnesium sulphate added in the assay of calcium lactate?
Replacement Titration: In this method the metal, which is to be analyzed, displaces quantitatively the metal from the complex. When direct or back titrations do not give sharp end points, the metal may be determined by the displacement of an equivalent amount of Mg or Zn from a less stable EDTA complex. Mn+2 + Mg EDTA---> 2 Mg+2 + Mn EDTA-2 Mn displaces Mg from Mn EDTA solution. The freed Mg metal is then directly titrated with a standard EDTA solution. In this method, excess quantity of Mg EDTA chelate is added to Mn solution. Mn quantitatively displaces Mg from Mg EDTA chelate. This displacement takes place because Mn forms a more stable complex with EDTA. By this method Ca, Pb, Hg may be determined using Eriochrome blackT indicator.
Is EDTA hexadentate ligand or tetradentate?
It is hexadentate strong field ligand a polydentate ligand is also known as chelating agent because it forms chelate like structure around the central metallic ion.
How do you calculate the concentration of EDTA in titration?
in order to titrate a sample of solution, lets take an example. If we have a solution of 1.569 mg of Coso4, which has a (155.0g/mol ratio) per mill. A question may ask us to find the volume of Edta needed of titrate an aliqout of this solution. So lets take a random number of 0.007840 M EDTA and be asked to titrate A 25.00ML Aliqout of this solution. How do we find the volume of EDTA needed.....? well first we use the numbers given, 1.569 mg CoSo4/ ml x (1g/1000mg)(1molcoso4/155.0g)(1molEDTA/1mol CoSo4) calculating this out should give 1.012 x 10 ^-5 mol of EDTA per ml. we then multuply the moles of EDTA which react with 1.569 ml of COso4 by 25.00 ml 1.012x10^-5 mol edta (25.00ml)= 2.531 x 10^-4 mol of edta. This is the amount of moles in the new solution. Now we need to find the amount of moles per liter of the specific concentration of EDTA. so we multiply 2.531x10^-4 mol edta x (1L/0.007840 mol) to give 0.03228 Liters of 32.28 ml .
Why do you use Fe-EDTA in murashige and skoog medium?
Iron is an essential plant nutrient and the EDTA is used to chelate (bind to) the iron to prevent it from precipitating in the solution.
How do you prepare 5mM EDTA of 100ml solution?
5mM = 0.005 moles 100 mL = 0.1 Liters Molarity = moles of solute/Liters of solution 0.005 M EDTA = X moles/0.1 Liters = 0.0005 moles EDTA =_____________ Now, look up the molecular formula for EDTA and find how many grams needed to add to your 100 mL.
How much quantity of EDTA dissolved in 1000ml to get 0.01 m solution of EDTA?
1 mole EDTA has 4 equivalents. 0.01 N EDTA = 0.01 equivalents/liter, so you need 0.01/4 = 0.0025 moles EDTA/liter.
Why is magnesium sulphate added in the assay of calcium lactate?
Replacement Titration: In this method the metal, which is to be analyzed, displaces quantitatively the metal from the complex. When direct or back titrations do not give sharp end points, the metal may be determined by the displacement of an equivalent amount of Mg or Zn from a less stable EDTA complex. Mn+2 + Mg EDTA---> 2 Mg+2 + Mn EDTA-2 Mn displaces Mg from Mn EDTA solution. The freed Mg metal is then directly titrated with a standard EDTA solution. In this method, excess quantity of Mg EDTA chelate is added to Mn solution. Mn quantitatively displaces Mg from Mg EDTA chelate. This displacement takes place because Mn forms a more stable complex with EDTA. By this method Ca, Pb, Hg may be determined using Eriochrome blackT indicator.
Is EDTA hexadentate ligand or tetradentate?
It is hexadentate strong field ligand a polydentate ligand is also known as chelating agent because it forms chelate like structure around the central metallic ion.
What does chelate do?
Although EDTA binds harmful, toxic metals like Mercury, lead, and cadmium, it also binds some essential nutrients of the body, such as copper, iron, calcium, zinc, and magnesium.
How do you calculate the concentration of EDTA in titration?
in order to titrate a sample of solution, lets take an example. If we have a solution of 1.569 mg of Coso4, which has a (155.0g/mol ratio) per mill. A question may ask us to find the volume of Edta needed of titrate an aliqout of this solution. So lets take a random number of 0.007840 M EDTA and be asked to titrate A 25.00ML Aliqout of this solution. How do we find the volume of EDTA needed.....? well first we use the numbers given, 1.569 mg CoSo4/ ml x (1g/1000mg)(1molcoso4/155.0g)(1molEDTA/1mol CoSo4) calculating this out should give 1.012 x 10 ^-5 mol of EDTA per ml. we then multuply the moles of EDTA which react with 1.569 ml of COso4 by 25.00 ml 1.012x10^-5 mol edta (25.00ml)= 2.531 x 10^-4 mol of edta. This is the amount of moles in the new solution. Now we need to find the amount of moles per liter of the specific concentration of EDTA. so we multiply 2.531x10^-4 mol edta x (1L/0.007840 mol) to give 0.03228 Liters of 32.28 ml .
Estimation of the amount of Zn present in the given solution of ZnCl2 using 0.01M EDTA solution.?
The amount of zinc is 0,01 moles.
What is molecular weight of edta?
To accurately calculate the molarity of an EDTA solution you need to determine the number of moles present and liters of solution. Take these two totals and divide them to determine molarity.
Why are multidentate ligands preferred to unidentate ligands in complexometric titrations?
Entropic advantage: [Cu(H2O)6]2+ + EDTA <==>> [Cu(EDTA)]2+ +6H2O Count your moles: Left side, 2 moles. Right side, 7 moles. The universe like to move from order to disorder. :-) Think of the occupy wall street people who would be happier if money all bundled up in some rich person's bank was distributed to the poor...The second law of thermodynamics applied to politics!
What does EDTA do exactly in plasmid preparation?
EDTA has high affinity towards divalent ions like Ca2+, Mn2+, Mg2+ which are cofactors for many active enzymes inside the cells. That includes nucleases which digests DNA molecules. Once the cell is disrupted, nuclear envelope goes off and the nuclear content comes into contact with the cellular content which is rich in nucleases. So the broken cell is treated with EDTA to chelate the ions so that nucleases loose their function and that we are able to get good yield of DNA.
Why EDTA is not dissolved in water?
EDTA is soluble in water.
