Malachite specimen A likely has smaller, more numerous crystals due to its quicker cooling rate. Rapid cooling can restrict crystal growth, resulting in finer crystals being formed. Additionally, the crystals in specimen A may be more prone to imperfections or defects compared to those in specimen B.
Methylene blue is added to a specimen primarily as a staining agent to enhance visibility under a microscope. It binds to cellular components, allowing for better contrast and differentiation of structures, which aids in the observation of cellular morphology and pathology. Additionally, it can help in identifying specific cell types or diagnosing certain conditions by highlighting particular features.
For a scanning electron microscope, the most commonly used type of EM, there is no need to do anything other than stick it to an electrically conducting pad. If the sample is particularly insulating, it may be worth coating it with a very thin gold layer to prevent the specimen from charging up with electrons from the beam (this would blur the image) For a transmission electron microscope, the specimen is most commonly encased in epoxy resin before being sliced into thin enough slices that the electron beam can pass through. The sample is then placed on a fine copper grid before being placed in the microscope.